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Induction studies with Escherichia coli expressing recombinant interleukin-13 using multi-parameter flow cytometry

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journal contribution
posted on 08.01.2010, 12:44 authored by Jennifer O. Shitu, John M. Woodley, Richard Wnek, Michel J. Chartrain, Christopher Hewitt
The expression of interleukin -13 following induction with IPTG in E. coli results in metabolic changes as indicated by multi-parameter flow cytometry and traditional methods of fermentation profiling (O2 uptake rate, CO2 evolution rate and optical density measurements). Induction early in the rapid growth phase was optimal although this led to lower overall biomass concentrations and lower maximum specific growth rates. In contrast, induction in the mid-rapid growth phase was the most detrimental to cell quality as measured by cytoplamsic membrane depolarisation.

History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Chemical Engineering

Citation

SHITU, J.O. ... et al, 2009. Induction studies with Escherichia coli expressing recombinant interleukin-13 using multi-parameter flow cytometry. Biotechnology Letters, 31 (4), pp. 577-584.

Publisher

© Springer Verlag

Version

AM (Accepted Manuscript)

Publication date

2009

Notes

This article was published in the journal, Biotechnology Letters [© Springer Verlag] and the original publication is available at www.springerlink.com

ISSN

0141-5492;1573-6776

Language

en