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Investigation of fibroblast and keratinocyte cell-scaffold interactions using a novel 3D cell culture system

journal contribution
posted on 12.05.2017, 12:34 by Tao SunTao Sun, David Norton, Anthony J. Ryan, Sheila MacNeil, John W. Haycock
In this study we investigated the influence of fibre diameter and interfibre space in 3D scaffolds on cellular behaviour of human dermal fibroblasts and a human keratinocyte cell line (HaCaT cell). Electrospun aligned poly L-lactic acid fibres (2–10 μ m) were bound to form fibres with a broad range of diameters (2–120 μ m) and then constructed in a specifically designed 3D cell culture system. Human dermal fibroblasts were introduced to one end of the free-standing fibres using a fibrin clot and encouraged to ‘walk the plank’. Under these conditions it was observed that a minimum fibre diameter of 10 μ m for fibroblast adhesion and migration arose. A thin layer of electrospun viscose rayon scaffold fibres (diameter 30–50 μ m, pore size 50–300 μ m) was also constructed in the 3D cell culture system. After introduction to the scaffold using cells contained within a fibrin clot, fibroblasts were observed to stratify and also elongate between fibres in order to occupy voids. An interfibre span of up to 200 μ m was possible by a single fibroblast, but more commonly void distances were spanned by cellular multilayering. In contrast, HaCaT keratinocytes cultured under identical conditions using viscose rayon scaffolds occupied very much smaller void distances of 50–80 μ m predominantly by stratification.

History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Chemical Engineering

Published in

Journal of Materials Science: Materials in Medicine

Volume

18

Issue

2

Pages

321 - 328

Citation

SUN, T. ... et al, 2007. Investigation of fibroblast and keratinocyte cell-scaffold interactions using a novel 3D cell culture system. Journal of Materials Science: Materials in Medicine, 18 (2), pp.321-328

Publisher

© Springer Science + Business Media, LLC

Version

NA (Not Applicable or Unknown)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2007

Notes

This paper is closed access.

ISSN

0957-4530

eISSN

1573-4838

Language

en

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