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Isolation of human fibrinogen by axial and radial flow chromatography from Nitschmann fraction I
journal contributionposted on 2017-05-12, 15:25 authored by Tao SunTao Sun, Ge Chen, Yipin Liu, Fengrong Bu, Meijuan Wen
An axial column (3×2.6 cm) and a radial flow column (3.5×5 cm) packed with DEAE Sepharose Fast Flow media had been evaluated for the separation of human fibrinogen. Nitschmann fraction I dissolved in buffered saline (0.015 M NaCl buffered with 0.06 M Tris/HCl to pH 7.5) was the starting material. Under radial flow conditions, sample flow up to 15 ml min−1 (i.e., 18 bed volumes h−1) was achieved. The operating pressures were below 0.2 MPa, even though the elution velocity was 30 ml min−1 (i.e., 36 bed volumes h−1).
- Aeronautical, Automotive, Chemical and Materials Engineering
- Chemical Engineering
Published inBiotechnology Techniques
Pages831 - 835
CitationSUN, T. ... et al, 1999. Isolation of human fibrinogen by axial and radial flow chromatography from Nitschmann fraction I. Biotechnology Techniques, 13 (12), pp.831-835
PublisherSpringer Verlag (© Kluwer Academic Publishers)
- NA (Not Applicable or Unknown)
Publisher statementThis work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/
NotesThis paper is closed access.