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Isolation of human fibrinogen by axial and radial flow chromatography from Nitschmann fraction I

journal contribution
posted on 12.05.2017, 15:25 by Tao SunTao Sun, Ge Chen, Yipin Liu, Fengrong Bu, Meijuan Wen
An axial column (3×2.6 cm) and a radial flow column (3.5×5 cm) packed with DEAE Sepharose Fast Flow media had been evaluated for the separation of human fibrinogen. Nitschmann fraction I dissolved in buffered saline (0.015 M NaCl buffered with 0.06 M Tris/HCl to pH 7.5) was the starting material. Under radial flow conditions, sample flow up to 15 ml min−1 (i.e., 18 bed volumes h−1) was achieved. The operating pressures were below 0.2 MPa, even though the elution velocity was 30 ml min−1 (i.e., 36 bed volumes h−1).

History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Chemical Engineering

Published in

Biotechnology Techniques

Volume

13

Pages

831 - 835

Citation

SUN, T. ... et al, 1999. Isolation of human fibrinogen by axial and radial flow chromatography from Nitschmann fraction I. Biotechnology Techniques, 13 (12), pp.831-835

Publisher

Springer Verlag (© Kluwer Academic Publishers)

Version

NA (Not Applicable or Unknown)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

1999

Notes

This paper is closed access.

ISSN

0951-208X

eISSN

1573-6784

Language

en