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Keeping an eye on decellularized corneas: a review of methods, characterization and applications
journal contribution
posted on 2017-10-06, 09:50 authored by Sammy WilsonSammy Wilson, Laura E. Sidney, Siobhan E. Dunphy, James B. Rose, Andrew HopkinsonThe worldwide limited availability of suitable corneal donor tissue has led to the development of alternatives, including keratoprostheses (Kpros) and tissue engineered (TE) constructs. Despite advances in bioscaffold design, there is yet to be a corneal equivalent that effectively mimics both the native tissue ultrastructure and biomechanical properties. Human decellularized corneas (DCs) could offer a safe, sustainable source of corneal tissue, increasing the donor pool and potentially reducing the risk of immune rejection after corneal graft surgery. Appropriate, human-specific, decellularization techniques and high-resolution, non-destructive analysis systems are required to ensure reproducible outputs can be achieved. If robust treatment and characterization processes can be developed, DCs could offer a supplement to the donor corneal pool, alongside superior cell culture systems for pharmacology, toxicology and drug discovery studies.
Funding
Funding from E-TERM Engineering, Tissue Engineering and Regenerative Medicine (Grant number: EP/I017801/1) are gratefully acknowledged.
History
School
- Mechanical, Electrical and Manufacturing Engineering
Published in
Journal of Functional BiomaterialsVolume
4Issue
3Pages
114 - 161Citation
WILSON, S. ... et al., 2013. Keeping an eye on decellularized corneas: a review of methods, characterization and applications. Journal of Functional Biomaterials, 4 (3), pp.114-161.Publisher
Multidisciplinary Digital Publishing Institute (© the authors)Version
- VoR (Version of Record)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution 3.0 Unported (CC BY 3.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/by/3.0/Publication date
2013ISSN
2079-4983Publisher version
Language
- en