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Keeping an eye on decellularized corneas: a review of methods, characterization and applications

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journal contribution
posted on 06.10.2017, 09:50 by Sammy WilsonSammy Wilson, Laura E. Sidney, Siobhan E. Dunphy, James B. Rose, Andrew Hopkinson
The worldwide limited availability of suitable corneal donor tissue has led to the development of alternatives, including keratoprostheses (Kpros) and tissue engineered (TE) constructs. Despite advances in bioscaffold design, there is yet to be a corneal equivalent that effectively mimics both the native tissue ultrastructure and biomechanical properties. Human decellularized corneas (DCs) could offer a safe, sustainable source of corneal tissue, increasing the donor pool and potentially reducing the risk of immune rejection after corneal graft surgery. Appropriate, human-specific, decellularization techniques and high-resolution, non-destructive analysis systems are required to ensure reproducible outputs can be achieved. If robust treatment and characterization processes can be developed, DCs could offer a supplement to the donor corneal pool, alongside superior cell culture systems for pharmacology, toxicology and drug discovery studies.

Funding

Funding from E-TERM Engineering, Tissue Engineering and Regenerative Medicine (Grant number: EP/I017801/1) are gratefully acknowledged.

History

School

  • Mechanical, Electrical and Manufacturing Engineering

Published in

Journal of Functional Biomaterials

Volume

4

Issue

3

Pages

114 - 161

Citation

WILSON, S. ... et al., 2013. Keeping an eye on decellularized corneas: a review of methods, characterization and applications. Journal of Functional Biomaterials, 4 (3), pp.114-161.

Publisher

Multidisciplinary Digital Publishing Institute (© the authors)

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution 3.0 Unported (CC BY 3.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/by/3.0/

Publication date

2013

ISSN

2079-4983

Language

en

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