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Download fileLab on a stick: Multi-analyte cellular assays in a microfluidic dipstick
journal contribution
posted on 2016-09-29, 10:06 authored by Nuno Reis, Jeremy Pivetal, Ana L. Loo-Zazueta, Joao M. S. Barros, Alexander D. Edwards© The Royal Society of Chemistry 2016.A new microfluidic concept for multi-analyte testing in a dipstick format is presented, termed "Lab-on-a-Stick", that combines the simplicity of dipstick tests with the high performance of microfluidic devices. Lab-on-a-stick tests are ideally suited to analysis of particulate samples such as mammalian or bacterial cells, and capable of performing multiple different parallel microfluidic assays when dipped into a single sample with results recorded optically. The utility of this new diagnostics format was demonstrated by performing three types of multiplex cellular assays that are challenging to perform in conventional dipsticks: 1) instantaneous ABO blood typing; 2) microbial identification; and 3) antibiotic minimum inhibitory (MIC) concentration measurement. A pressure balance model closely predicted the superficial flow velocities in individual capillaries, that were overestimated by up to one order of magnitude by the Lucas-Washburn equation conventionally used for wicking in cylindrical pores. Lab-on-a-stick provides a cost-effective, simple, portable and flexible multiplex platform for a range of assays, and will deliver a new generation of advanced yet affordable point-of-care tests for global diagnostics.
Funding
This work was funded by EPSRC (grant EP/L013983/1).
History
School
- Aeronautical, Automotive, Chemical and Materials Engineering
Department
- Chemical Engineering
Published in
Lab on a Chip - Miniaturisation for Chemistry and BiologyVolume
16Issue
15Pages
2891 - 2899Citation
REIS, N.M. ...et al., 2016. Lab on a stick: Multi-analyte cellular assays in a microfluidic dipstick. Lab on a Chip - Miniaturisation for Chemistry and Biology, 16 (15), pp. 2891-2899.Publisher
© The Authors. Published by the Royal Society of Chemistry.Version
- VoR (Version of Record)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution 3.0 Unported (CC BY 3.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/by/3.0/Publication date
2016-06-23Notes
This is an Open Access Article. It is published by Royal Society of Chemistry under the Creative Commons Attribution 3.0 Unported Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/3.0/ISSN
1473-0197eISSN
1473-0189Publisher version
Language
- en