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Download fileLiquid crystalline ordered collagen substrates for applications in tissue engineering
journal contribution
posted on 2017-01-25, 10:01 authored by Joshua C. Price, Paul RoachPaul Roach, Alicia J. El HajThis report describes methods for fabricating
substrates with anisotropic order from a single solution of high concentration collagen. By exploiting the intrinsic
property of collagen to behave as a cholesteric liquid crystal, we demonstrate first the production of dense collagen films
containing anisotropic fibers by simple dialysis and polymerization in ammonia vapor. We then utilized shear driven
alignment of collagen using viscous extrusion to produce aligned collagen fibers. Next we describe an evaporation
technique to observe crystalline growth into the collagen, which serves to template the substrate prior to fibrillogenesis. The
ordered substrates supported osteogenic differentiation of hMSCs and also oriented growth of hMSCs. We also demonstrate using Raman spectroscopy that the local protein
concentration in the substrates influenced the molecular orientation of collagen, Finally, we compare the resultant textures in the substrates with section of native cornea and tendon using polarized light microscopy, which showed remarkable similarities in terms of both anisotropy and second order chiral structure. These rapid, cost-effective methods could potentially serve a range of different applications in tissue engineering.
Funding
This research was funded by EPSRC DTC in Regenerative medicine, Keele University. Grant funding number: EP/F500491/1
History
School
- Science
Department
- Physics
Published in
ACS Biomaterials Science & EngineeringVolume
2Issue
4Pages
625 - 633Citation
PRICE, J.C., ROACH, P. and EL HAJ, A.J., 2016. Liquid crystalline ordered collagen substrates for applications in tissue engineering. ACS Biomaterials Science & Engineering, 2(4), pp. 625-633.Publisher
© American Chemical SocietyVersion
- NA (Not Applicable or Unknown)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Acceptance date
2016-03-03Publication date
2016-03-03Copyright date
2016Notes
This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Biomaterials Science & Engineering, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acsbiomaterials.6b00030ISSN
2373-9878eISSN
2373-9878Publisher version
Language
- en