Liquid crystalline ordered collagen substrates for applications in tissue engineering
journal contributionposted on 2017-01-25, 10:01 authored by Joshua C. Price, Paul RoachPaul Roach, Alicia J. El Haj
This report describes methods for fabricating substrates with anisotropic order from a single solution of high concentration collagen. By exploiting the intrinsic property of collagen to behave as a cholesteric liquid crystal, we demonstrate first the production of dense collagen films containing anisotropic fibers by simple dialysis and polymerization in ammonia vapor. We then utilized shear driven alignment of collagen using viscous extrusion to produce aligned collagen fibers. Next we describe an evaporation technique to observe crystalline growth into the collagen, which serves to template the substrate prior to fibrillogenesis. The ordered substrates supported osteogenic differentiation of hMSCs and also oriented growth of hMSCs. We also demonstrate using Raman spectroscopy that the local protein concentration in the substrates influenced the molecular orientation of collagen, Finally, we compare the resultant textures in the substrates with section of native cornea and tendon using polarized light microscopy, which showed remarkable similarities in terms of both anisotropy and second order chiral structure. These rapid, cost-effective methods could potentially serve a range of different applications in tissue engineering.
This research was funded by EPSRC DTC in Regenerative medicine, Keele University. Grant funding number: EP/F500491/1
Published inACS Biomaterials Science & Engineering
Pages625 - 633
CitationPRICE, J.C., ROACH, P. and EL HAJ, A.J., 2016. Liquid crystalline ordered collagen substrates for applications in tissue engineering. ACS Biomaterials Science & Engineering, 2(4), pp. 625-633.
Publisher© American Chemical Society
- NA (Not Applicable or Unknown)
Publisher statementThis work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/
NotesThis document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Biomaterials Science & Engineering, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acsbiomaterials.6b00030