Localising individual atoms of tryptophan side chains in the metallo-β-lactamase IMP-1 by pseudocontact shifts from paramagnetic lanthanoid tags at multiple sites
posted on 2022-03-10, 14:50authored byHenry W Orton, Iresha D Herath, Ansis Maleckis, Shereen Jabar, Monika Szabo, Bim Graham, Colum Breen, Lydia Topping, Stephen ButlerStephen Butler, Gottfried Otting
The metallo-β-lactamase IMP-1 features a flexible loop near the active site that assumes different conformations in single crystal
structures, which may assist in substrate binding and enzymatic activity. To
probe the position of this loop, we labelled the tryptophan residues of
IMP-1 with 7-13C-indole and the protein with lanthanoid tags at three
different sites. The magnetic susceptibility anisotropy (Δχ)
tensors were determined by measuring pseudocontact shifts (PCSs) of backbone amide protons. The Δχ tensors were subsequently used to
identify the atomic coordinates of the tryptophan side chains in the
protein. The PCSs were sufficient to determine the location of Trp28, which
is in the active site loop targeted by our experiments, with high accuracy. Its average atomic coordinates showed barely significant changes in response to the inhibitor captopril. It was found that localisation spaces could be defined with better accuracy by including only the PCSs of a single paramagnetic lanthanoid ion for each tag and tagging site. The effect
was attributed to the shallow angle with which PCS isosurfaces tend to
intersect if generated by tags and tagging sites that are identical except
for the paramagnetic lanthanoid ion.
Funding
Australian Research Council (grant nos. CE200100012 and FL170100019)
European Regional Development Fund (grant no. 1.1.1.2/VIAA/2/18/381)
This is an Open Access Article. It is published by Copernicus Publications under the Creative Commons Attribution 4.0 International Licence (CC BY 4.0). Full details of this licence are available at: https://creativecommons.org/licenses/by/4.0/