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Loss of solubility of alpha-lactalbumin and beta-lactoglobulin during the spray drying of whey proteins

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posted on 2013-01-16, 09:41 authored by C. Anandharamakrishnan, Chris RiellyChris Rielly, Andy StapleyAndy Stapley
A reversed phase HPLC technique (at pH 4.6) has been developed to measure 10 the loss of solubility of α-lactalbumin and β-lactoglobulin resulting from the 11 spray drying of whey protein isolate solution. Spray drying was performed in a 12 pilot-scale co-current spray dryer with different feed concentrations (20 - 40% 13 w/v) and outlet temperatures (60°C to 120°C). The study reveals that the 14 solubility of both α-lactalbumin and β-lactoglobulin was not significantly affected 15 at low outlet gas temperatures (60-80°C), but was strongly affected (up to 40%) 16 at high temperatures (100-120°C). Significantly higher losses in solubility were 17 observed for β-lactoglobulin compared to α-lactalbumin. Increasing the feed 18 concentration at higher outlet temperatures also caused noticeable increases in 19 insolubility. The reversed phase HPLC results were consistent with those from 20 total protein nitrogen content (Kjeldhal) analysis.

History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Chemical Engineering

Citation

ANANDHARAMAKRISHNAN, C., RIELLY, C.D. and STAPLEY, A.G.F., 2008. Loss of solubility of alpha-lactalbumin and beta-lactoglobulin during the spray drying of whey proteins. LWT - Food Science and Technology, 41 (2), pp. 270-277.

Publisher

© Elsevier

Version

  • AM (Accepted Manuscript)

Publication date

2008

Notes

This is the author’s version of a work that was accepted for publication in the LWT - Food Science and Technology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published at: http://dx.doi.org/10.1016/j.lwt.2007.03.004

ISSN

0023-6438

Language

  • en

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