This is the peer reviewed version of the following article: MESQUITA, R.N.O., ŠKARABOT, J. and PEARCEY, G.E.P., 2020. Low‐threshold motor units can be a pain during experimental muscle pain. The Journal of Physiology, 598 (13), pp.2545-2547, which has been published in final form at https://doi.org/10.1113/JP279872. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.