Mechanically demanding eccentric exercise increases nuclear factor erythroid 2-related factor 2 activity in human peripheral blood mononuclear cells
This study examined whether eccentric exercise increases nuclear factor erythroid 2-related factor 2 (NRF2) activity in peripheral blood mononuclear cells (PBMCs). Twenty-six recreationally active males (mean [SD]; age 25  y, height 178.4 [6.9] cm, body mass 77.6 [7.4] kg) were allocated to either an exercise (n = 16) or non-exercise (resting) control (n = 10) group. Eccentric exercise involved performing 100 drop jumps from a 0.6 m box. Blood was collected pre-, immediately post- and 1 h post-exercise or rest. NRF2/antioxidant response element (ARE) binding was measured in PBMCs; glutathione reductase (GR) and peroxidase (GPX) were measured in plasma. NRF2/ARE binding was greater immediately post- and 1 h post in the exercise vs. rest group (p < 0.05 for all). NRF2/ARE binding was higher at 1 h post vs. post-exercise (fold change from baseline: [post] 1.31 ± 1.31, [1 h] 2.79 ± 3.30; p = 0.003). GR did not increase in response to exercise or rest (p = 0.377) but was higher at all time-points in the exercise vs resting group (p = 0.023). Exercise or rest had no impact on GPX activity (p > 0.05 for all). Eccentric exercise increases NRF2/ARE binding in PBMCs compared to rest.
- Sport, Exercise and Health Sciences
Published inJournal of Sports Sciences
PublisherInforma UK Limited, trading as Taylor & Francis Group
- VoR (Version of Record)
Rights holder© The Author(s)
Publisher statementThis is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.