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Microdroplet-based multiplex PCR on chip to detect foodborne bacteria producing biogenic amines

journal contribution
posted on 11.06.2015, 12:03 authored by Anna G. Sciancalepore, Elisa MeleElisa Mele, Valentina Arcadio, Francesco Reddavide, Francesco Grieco, Giuseppe Spano, Patrick Lucas, Giovanni Mita, Dario Pisignano
The development of fast, reliable and culture-independent molecular tools to detect bacteria producing biogenic amines deserves the attention of research and ultimately of the food industry in order to protect consumers’ health. Here we present the application of a simple, low-cost, fast and sensitive method to perform microdroplet-based multiplex PCR, directly on a food matrix, for the simultaneous detection of bacterial genes involved in biogenic amine biosynthesis. After inoculating wine with Lactobacillus brevis IOEB 9809, cell lysis and DNA amplification are performed in one single step, without preliminary nucleic acid extraction or purification treatments. The assay is performed in about 30 min, requiring 150 nL of starting sample and it enables the detection of down to 15 bacterial cells. With respect to traditional culture techniques, the speed, the simplicity and the cheapness of this procedure allow an effective monitoring of microbial cells during food-making and processing.

Funding

G.S. and P.L. were supported by the European Union project BIAMFOOD/211441 (Controlling Biogenic Amines in Traditional Food Fermentations in Regional Europe).

History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Materials

Published in

FOOD MICROBIOLOGY

Volume

35

Issue

1

Pages

10 - 14 (5)

Citation

SCIANCALEPORE, A.G. ... et al, 2013. Microdroplet-based multiplex PCR on chip to detect foodborne bacteria producing biogenic amines. Food Microbiology, 35 (1), pp. 10 - 14.

Publisher

© Elsevier Ltd

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2013

Notes

This article is closed access.

ISSN

0740-0020

Language

en