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Mimicking exercise in three-dimensional bioengineered skeletal muscle to investigate cellular and molecular mechanisms of physiological adaptation

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journal contribution
posted on 24.02.2017, 14:06 by Andreas M. Kasper, Daniel C. Turner, Neil MartinNeil Martin, Adam Sharples
Bioengineering of skeletal muscle in-vitro in order to produce highly aligned myofibres in relevant three dimensional (3D) matrices have allowed scientists to model the in-vivo skeletal muscle niche. This review discusses essential experimental considerations for developing bioengineered muscle in order to investigate exercise mimicking stimuli. We identify current knowledge in the use of electrical stimulation and co-culture with motor neurons to enhance skeletal muscle maturation and contractile function in bioengineered systems in-vitro. Importantly, we provide a current opinion on the use of acute and chronic exercise mimicking stimuli (electrical stimulation and mechanical overload) and the subsequent mechanisms underlying physiological adaptation in 3D bioengineered muscle. We also identify that future studies using the latest bioreactor technology, providing simultaneous electrical and mechanical loading and flow perfusion in-vitro, may provide the basis for advancing knowledge in the future. We also envisage, that more studies using genetic, pharmacological and hormonal modifications applied in human 3D bioengineered skeletal muscle may allow for an enhanced discovery of the in-depth mechanisms underlying the response to exercise in relevant human testing systems. Finally, 3D bioengineered skeletal muscle may provide an opportunity to be used as a pre-clinical in-vitro test-bed to investigate the mechanisms underlying catabolic disease, whilst modelling disease itself via the use of cells derived from human patients without exposing animals or humans (in phase I trials) to the side effects of potential therapies.

Funding

Authors would like to thank the Rugby Football Union, UK and the Society for Endocrinology for funding and supporting this work.

History

School

  • Sport, Exercise and Health Sciences

Published in

Journal of Cellular Physiology

Volume

233

Issue

3

Pages

1985–1998

Citation

KASPER, A.M. ... et al, 2017. Mimicking exercise in three-dimensional bioengineered skeletal muscle to investigate cellular and molecular mechanisms of physiological adaptation. Journal of Cellular Physiology, 233 (3), pp. 1985–1998.

Publisher

© Wiley

Version

AM (Accepted Manuscript)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Acceptance date

01/02/2017

Publication date

2017-02-03

Copyright date

2018

Notes

This is the peer reviewed version of the following article: KASPER, A.M. ... et al, 2017. Mimicking exercise in three-dimensional bioengineered skeletal muscle to investigate cellular and molecular mechanisms of physiological adaptation. Journal of Cellular Physiology, 233 (3), pp. 1985–1998, which has been published in final form at http://dx.doi.org/10.1002/jcp.25840. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

ISSN

0021-9541

eISSN

1097-4652

Language

en