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Molecular mechanisms of seed dormancy release by gas plasma-activated water technology

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posted on 2022-06-30, 13:32 authored by Giles Grainge, Kazumi Nakabayashi, Tina Steinbrecher, Sue Kennedy, Junchen Ren, Felipe IzaFelipe Iza, Gerhard Leubner-Metzger

Developing innovative agri-technologies is essential for the sustainable intensification of global food production. Seed dormancy is an adaptive trait which defines the environmental conditions in which the seed is able to germinate. Dormancy release requires sensing and integration of multiple environmental signals, a complex process which may be mimicked by seed treatment technologies. Here, we reveal molecular mechanisms by which non-thermal (cold) atmospheric gas plasma-activated water (GPAW) releases the physiological seed dormancy of Arabidopsis thaliana. GPAW triggered dormancy release by synergistic interaction between plasma-generated reactive chemical species (NO3–, H2O2, ·NO, and ·OH) and multiple signalling pathways targeting gibberellin and abscisic acid (ABA) metabolism and the expression of downstream cell wall-remodelling genes. Direct chemical action of GPAW on cell walls resulted in premature biomechanical endosperm weakening. The germination responses of dormancy signalling (nlp8, prt6, and dog1) and ABA metabolism (cyp707a2) mutants varied with GPAW composition. GPAW removes seed dormancy blocks by triggering multiple molecular signalling pathways combined with direct chemical tissue weakening to permit seed germination. Gas plasma technologies therefore improve seed quality by mimicking permissive environments in which sensing and integration of multiple signals lead to dormancy release and germination.

Funding

Gas plasma treatment as a novel seed technology to release dormancy and improve germination uniformity of vegetable seeds

Biotechnology and Biological Sciences Research Council

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13TSB_ACT Novel biomaterial engineering technologies, molecular and hormone analyses to improve lettuce seed priming and production in stressful envir

Biotechnology and Biological Sciences Research Council

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PlasSeed: Gas Plasma for Seed Disinfection

Biotechnology and Biological Sciences Research Council

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PlasSeed: Gas Plasma for Seed Disinfection

Biotechnology and Biological Sciences Research Council

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History

School

  • Mechanical, Electrical and Manufacturing Engineering

Published in

Journal of Experimental Botany

Volume

73

Issue

12

Pages

4065 - 4078

Publisher

Oxford University Press

Version

  • VoR (Version of Record)

Rights holder

© The Authors

Publisher statement

This is an Open Access Article. It is published by Oxford University Press under the Creative Commons Attribution 4.0 International Licence (CC BY). Full details of this licence are available at: https://creativecommons.org/licenses/by/4.0/

Acceptance date

2022-04-13

Publication date

2022-04-15

Copyright date

2022

ISSN

0022-0957

eISSN

1460-2431

Language

  • en

Depositor

Deposit date: 28 June 2022