Monochromatic computed microtomography using laboratory and synchrotron sources and X-ray fluorescence analysis for comprehensive analysis of structural changes in bones

Buzmakov, Alexey; Chukalina, Marina; Nikolaev, Dmitry; Gulimova, Victoriya; Savel'ev, Sergey; Tereschenko, Elena; Seregin, Alexey; Senin, Roman; Zolotov, Denis; Prun, Victor; Shaefer, Gerald; Asadchikov, Victor

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Monochromatic computed microtomography using laboratory and synchrotron sources and X-ray fluorescence analysis for comprehensive analysis of structural changes in bones

journal contribution

posted on 2015-08-18, 13:26 authored by Alexey Buzmakov, Marina Chukalina, Dmitry Nikolaev, Victoriya Gulimova, Sergey Savel'ev, Elena Tereschenko, Alexey Seregin, Roman Senin, Denis Zolotov, Victor Prun, Gerald Shaefer, Victor Asadchikov

A combination of X-ray tomography at different wavelengths and X-ray fluorescence analysis was applied in the study of two types of bone tissue changes: prolonged presence in microgravity conditions and age-related bone growth. The proximal tail vertebrae of geckos were selected for investigation because they do not bear the supporting load in locomotion, which allows them to be considered as an independent indicator of gravitational influence. For the vertebrae of geckos no significant differences were revealed in the elemental composition of the flight samples and the synchronous control samples. In addition, the gecko bone tissue samples from the jaw apparatus, spine and shoulder girdle were measured. The dynamics of structural changes in the bone tissue growth was studied using samples of a human fetal hand. The hands of human fetuses of 11–15 weeks were studied. Autonomous zones of calcium accumulation were found not only in individual fingers but in each of the investigated phalanges. The results obtained are discussed.

Funding

This study was supported by the grants of the RFBR 13-02- 00552, 13-07-00970, 13-07-12179 and by the Russian Ministry of Education and Science (agreement No. 14.616.21.0005). The investigations were partially performed using the equipment of the Shared Research Center of the Shubnikov Institute of Crystallography RAS and were supported by the Russian Ministry of Education and Science (project RFMEFI62114X0005), and the Mixed-Use Center of the Kurchatov Center of Synchrotron Radiation and Nanotechnologies (state contract No. 16.552.11.7003).

History

School

Science

Department

Computer Science

Published in

JOURNAL OF APPLIED CRYSTALLOGRAPHY

Volume

48

Pages

693 - 701 (9)

Citation

BUZMAKOV, A. ... et al, 2015. Monochromatic computed microtomography using laboratory and synchrotron sources and X-ray fluorescence analysis for comprehensive analysis of structural changes in bones. Journal of Applied Crystallography, 48, pp. 693 - 701.

Publisher

International Union of Crystallography

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution 3.0 Unported (CC BY 3.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/by/3.0/

Publication date

2015

Notes

This is an Open Access article published by the International Union of Crystallography and distributed under the terms of the Creative Commons Attribution Licence.