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Multi-parameter flow cytometry and cell sorting reveal extensive physiological heterogeneity in Bacillus cereus batch cultures.

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journal contribution
posted on 14.07.2011, 16:13 authored by Andrew Want, Helen Hancocks, Stuart M. Stocks, Gerhard Nebe-von-Caron, Christopher Hewitt
Based on two staining protocols, DiOC6(3)/ propidium iodide (PI) and RedoxSensor Green (an indicator of bacterial reductase activity)/PI, multiparameter flow cytometry and cell sorting has identified at least four distinguishable physiological states during batch cultures of Bacillus cereus. Furthermore, dependent on the position in the growth curve, single cells gave rise to varying numbers of colonies when sorted individually onto nutrient agar plates. These growing colonies derived from a single cell had widely different lag phases, inferred fromdifferences in colony size.This further highlights the complex population dynamics of bacterial monocultures and further demonstrates that individual bacterial cells in a culture respond in markedly dissimilar ways to the environment, resulting in a physiologically heterogenous and dynamic population.

History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Chemical Engineering

Citation

WANT, A. ... et al, 2011. Multi-parameter flow cytometry and cell sorting reveal extensive physiological heterogeneity in Bacillus cereus batch cultures. Biotechnology Letters, 33 (7), pp. 1395–1405.

Publisher

© Springer

Version

AM (Accepted Manuscript)

Publication date

2011

Notes

The final publication is available at http://www.springerlink.com

ISSN

0141-5492;1573-6776

Language

en