Mabrouk et al_IJP_MNP_Revised manuscript_cleaned_11-07-2020.pdf (1.18 MB)
Multifunctional magnetite nanoparticles for drug delivery: preparation, characterisation, antibacterial properties and drug release kinetics
journal contribution
posted on 2020-07-14, 11:20 authored by Mostafa Mabrouk, Rasha M Abd El-Wahab, Hanan H Beherei, Mohamed M Selim, Diganta DasDiganta DasMultifunctional nanoparticles (NPs) with magnetic (M) and antibacterial properties were prepared for drug delivery purposes by a method involving co-precipitation synthesis. Partial and complete substitutions of ferrous ions (Fe2+) by copper ions (Cu2+) were carried out for the preparation of the magnetite NPs, which are designated as Cu0.5M and CuM, respectively, in this work. In addition, chitosan and ciprofloxacin were hybridized with the NPs from the previous step to achieve multifunctional properties. XRD, TEM, SEM/EDAX, VSM and FTIR were subsequently employed to characterize various properties of the prepared NPs, namely, crystallinity, nanostructure (size), particle morphology, elemental mapping, magnetic strength and chemical composition. Antibacterial properties of the NPs were tested against Bacillus cereus (Gram-positive bacteria), Escherichia coli (Gram-negative bacteria) and Candida albicans (yeast). Efficiency of the ciprofloxacin release was also studied for the drug-loaded NPs. It is demonstrated that the obtained NPs possess mixed phases with crystalline structures that are affected by the degree of Cu ion substitution (5-10nm (M), 2.5-3.5nm (Cu0.5M) and 11-16nm (CuM)). Saturation magnetization values of the NPs were recorded as 38.7, 3.5 and 1.3 emu/g, respectively. It was also found that the introduction of Cu ions in the NP samples improved the significance of their antibacterial activity, especially against Escherichia coli. Chitosan and ciprofloxacin were found to have stronger effects against Bacillus cereus and Escherichia coli and lesser effects against Candida albicans. However, the samples containing chitosan, ciprofloxacin and the higher Cu ion concentration exhibited strong influence against Candida albicans. During a study period of 30- days, the amounts of released drug from the tested NPs were 85, 26 and 20% of the originally loaded amount, respectively. Owing to the findings in this paper, the developed NPs are considered to have good potential for drug delivery applications and to study them further such as in pre-clinical studies.
History
School
- Aeronautical, Automotive, Chemical and Materials Engineering
Department
- Chemical Engineering
Published in
International Journal of PharmaceuticsVolume
587Publisher
ElsevierVersion
- AM (Accepted Manuscript)
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© Elsevier B.V.Publisher statement
This paper was accepted for publication in the journal International Journal of Pharmaceutics and the definitive published version is available at https://doi.org/10.1016/j.ijpharm.2020.119658.Acceptance date
2020-07-13Publication date
2020-07-16Copyright date
2020ISSN
0378-5173Publisher version
Language
- en
Depositor
Dr Diganta Das. Deposit date: 13 July 2020Article number
119658Usage metrics
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