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Multiparameter flow cytometry for the characterisation of extracellular markers on human mesenchymal stem cells

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journal contribution
posted on 16.06.2014, 13:28 by Alexander K.C. Chan, Thomas R.J. Heathman, Karen Coopman, Christopher Hewitt
Extracellular surface proteins are used to identify fully-functional human mesenchymal stem cells (hMSCs) in a mixed population. Here, a multiparameter flow cytometry assay was developed to examine the expression of several bone marrow-derived hMSC markers simultaneously at the single cell level. The multiparameter approach demonstrates a depth of analysis that goes far beyond the conventional single or dual staining methods. CD73, CD90 and CD105 were chosen as positive markers as they are expressed on multipotent hMSCs, whilst CD34 and HLA-DR were chosen as negative indicators. Single colour analysis suggested a population purity of 100 %; in contrast, when analysed via the multiparameter method, the CD73/CD105/CD90/HLA-DR/CD34 phenotypes represented 94.5 ± 1.3 % of the total cell population. Also, although CD271 has been posited as a definite early stage hMSC marker, here we show it is not present on pre-passage cells, highlighting the need for careful marker selection. © 2013 Springer Science+Business Media Dordrecht.

Funding

The authors acknowledge funding support from the Engineering and Physical Sciences Research Council (EPSRC) Doctoral Training Centre in Regenerative Medicine and Bioprocessing Research Industries Club (BRIC).

History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Chemical Engineering

Published in

Biotechnology Letters Biotechnology Letters

Volume

36

Issue

4

Pages

731 - 741

Citation

CHAN, A.K.C. ... et al, 2014. Multiparameter flow cytometry for the characterisation of extracellular markers on human mesenchymal stem cells. Biotechnology Letters, 36 (4), pp. 731 - 741

Publisher

© Springer Science+Business Media

Version

AM (Accepted Manuscript)

Publication date

2014

Notes

The final publication is available at Springer via http://dx.doi.org/10.1007/s10529-013-1422-0

ISSN

0141-5492

eISSN

1573-6776

Language

en

Exports

Loughborough Publications

Exports