Multiplexed droplet Interface bilayer formation

Barlow, Nathan E.; Bolognesi, Guido; Flemming, Anthony J.; Brooks, Nicholas J.; Barter, Laura M.C.; Ces, Oscar

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Multiplexed droplet Interface bilayer formation

journal contribution

posted on 15.12.2016, 13:51 by Nathan E. Barlow, Guido BolognesiGuido Bolognesi, Anthony J. Flemming, Nicholas J. Brooks, Laura M.C. Barter, Oscar Ces

We present a simple method for the multiplexed formation of droplet interface bilayers (DIBs) using a mechanically operated linear acrylic chamber array. To demonstrate the functionality of the chip design, a lipid membrane permeability assay is performed. We show that multiple, symmetric DIBs can be created and separated using this robust low-cost approach.

Funding

The research leading to these results has received funding from the People Programme (Marie Curie Actions) of the European Union's Seventh Framework Programme (FP7/2007-2013) under REA grant agreement no 607466. This research was funded by EPSRC grants: EP/J017566/1, EP/L015498/1, EP/ J021199/1 and EP/K503733/1.

History

School

Aeronautical, Automotive, Chemical and Materials Engineering

Department

Chemical Engineering

Published in

Lab Chip

Volume

16

Issue

24

Pages

4653 - 4657

Citation

BARLOW, N.E. ... et al, 2016. Multiplexed droplet Interface bilayer formation. Lab Chip, 16 (24), pp. 4653 - 4657.

Publisher

Royal Society of Chemistry

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution 3.0 Unported (CC BY 3.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/by/3.0/

Acceptance date

02/11/2016

Publication date

2016-11-10

Copyright date

2016

Notes

This is an open access article published by the Royal Society of Chemistry and distributed under the terms of the Creative Commons Attribution Licence (CC BY 3.0), https://creativecommons.org/licenses/by/3.0/