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Neural and aneural regions generated by the use of chemical surface coatings

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journal contribution
posted on 26.01.2018, 09:37 by Maria M. Pardo-Figuerez, Neil Martin, Darren J. Player, Andrew Capel, Steven Christie, Mark Lewis
The disordered environment found in conventional neural cultures impedes various applications where cell directionality is a key process for functionality. Neurons are highly specialized cells known to be greatly dependent on interactions with their surroundings. Therefore, when chemical cues are incorporated on the surface material, a precise control over neuronal behavior can be achieved. Here, the behavior of SH-SY5Y neurons on a variety of self-assembled monolayers (SAMs) and polymer brushes both in isolation and combination to promote cellular spatial control was determined. APTES and BIBB coatings promoted the highest cell viability, proliferation, metabolic activity, and neuronal maturation, while low cell survival was seen on PKSPMA and PMETAC surfaces. These cell-attractive and repulsive surfaces were combined to generate a binary BIBB-PKSPMA coating, whereby cellular growth was restricted to an exclusive neural region. The utility of these coatings when precisely combined could act as a bioactive/bioinert surface resulting in a biomimetic environment where control over neuronal growth and directionality can be achieved.

Funding

We thank Loughborough University and EPSRC (Grant EP/L02067X/1) for financially supporting this project.

History

School

  • Sport, Exercise and Health Sciences

Published in

ACS Biomaterials Science and Engineering

Volume

4

Issue

1

Pages

98-106

Citation

PARDO-FIGUEREZ, M.M. ... et al, 2017. Neural and aneural regions generated by the use of chemical surface coatings. ACS Biomaterials Science and Engineering, 4 (1), pp. 98–106.

Publisher

© American Chemical Society

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution 4.0 International (CC BY 4.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/ by/4.0/

Acceptance date

29/11/2017

Publication date

2017-12-14

Copyright date

2018

Notes

This is an Open Access Article. It is published by the American Chemical Society under the Creative Commons Attribution 4.0 International Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/

ISSN

2373-9878

Language

en

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