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New solid-state fermentation process for repeated batch production of fibrinolytic enzyme by Fusarium oxysporum
journal contributionposted on 2017-05-12, 15:46 authored by Tao SunTao Sun, Beihui Liu, Peng Li, Deming Liu, Zuohu Li
novel solid-state fermentation system using rice chaff as carrier/substrate impregnated with mineral solution was developed in order to produce, repeatedly, an industrially useful enzyme. Fusarium oxysporum and a fibrinolytic enzyme was used as the model microorganism and target product, respectively. The dry spores were pneumatically inoculated and incubated for 96 hours, resulting in immobilized F. oxysporum cells on moist substrate. The ability of the immobilized culture to perform a long-term operation was confirmed by repeated batch fermentation. Production of the enzyme continued for 31.5 days and total fibrinolytic enzyme activity reached 154500 IU/litre bulk volume.
- Aeronautical, Automotive, Chemical and Materials Engineering
- Chemical Engineering
Published inProcess Biochemistry
Pages419 - 422
CitationSUN, T. ... et al, 1998. New solid-state fermentation process for repeated batch production of fibrinolytic enzyme by Fusarium oxysporum. Process Biochemistry, 33 (4), pp.419-422
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Publisher statementThis work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/
NotesThis paper is closed access.