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Download fileOptimization of sample preparation using glass slides for spectral pathology
journal contribution
posted on 2021-03-23, 11:46 authored by Lewis M. Dowling, Paul RoachPaul Roach, Abigail V. Rutter, Ibraheem Yousef, Srinivas Pilla, Deborah Latham, Daniel G. van Pittius, Josep Sulé-SusoThe clinical translation of Fourier transform infrared (FT-IR) microspectroscopy in pathology will require bringing this technique as close as possible to standard practice in pathology departments. An important step is sample preparation for both FT-IR microspectroscopy and pathology. This should entail minimal disruption of standard clinical practice while achieving good quality FT-IR spectral data. In fact, the recently described possibility of obtaining FT-IR spectra of cells placed on glass substrates brings FT-IR microspectroscopy closer to a clinical application. We have now furthered this work in order to identify two different types of lung cancer cells placed on glass coverslips. Two types of sample preparation which are widely used in pathology, cytospin and smear, have been used. Samples were fixed with either methanol, used in pathology, or formalin (4% paraformaldehyde) used widely in spectroscopy. Fixation with methanol (alcohol-based fixative) removed lipids from cells causing a decrease in intensity of the peaks at 2850 cm−1 and 2920 cm−1. Nevertheless, we show for the first time that using either type of sample preparation and fixation on thin glass coverslips allowed to differentiate between two different types of lung cancer cells using either the lipid region or the fingerprint region ranging from 1800 cm−1 to 1350 cm−1. We believe that formalin-fixed cytospin samples would be preferred to study cells on thin coverslips using FT-IR microspectroscopy. This work presents a clear indication for future advances in clinical assessment of samples within pathology units to gain a deeper understanding of cells/tissues under investigation.
Funding
Cancer Centre and University Hospitals of North Midlands charities
Alba Synchrotron Light Source
History
School
- Science
Department
- Chemistry
Published in
Applied SpectroscopyVolume
75Issue
3Pages
343 - 350Publisher
SAGE PublicationsVersion
- VoR (Version of Record)
Rights holder
© The AuthorsPublisher statement
This is an Open Access Article. It is published by SAGE under the Creative Commons Attribution-NonCommercial 4.0 International Licence (CC BY-NC 4.0). Full details of this licence are available at: https://creativecommons.org/licenses/by-nc/4.0/Acceptance date
2020-06-30Publication date
2020-07-14Copyright date
2020ISSN
0003-7028eISSN
1943-3530Publisher version
Language
- en