Photo-crosslinkable microcapsules formed by polyelectrolyte copolymer and modified collagen for rat hepatocyte encapsulation
journal contribution
posted on 2017-05-12, 14:07authored byChai-Hoon Quek, Jun Li, Tao SunTao Sun, Melinda Ling Hou Chan, Hai-Quan Mao, Leong Ming Gan, Kam W. Leong, Hanry Yu
New anionic polyelectrolyte tetra-copolymers with photo-crosslinkable 4-(4-methoxycinnamoyl)phenyl methacrylate monomer in addition to a HEMA-MMA-MAA ter-copolymer system were synthesized. The tetra-copolymers were used to form photo-crosslinkable microcapsules with modified collagen by complex coacervation for rat hepatocytes encapsulation. The hepatocytes were encapsulated within a two-layered membrane comprising of modified collagen as the inner core and an outer photo-crosslinkable copolymer shell. Upon photo-crosslinking of the microcapsules with UV–Vis light irradiation, the mechanical strength and chemical stability of the microcapsules, and the cellular functions of the encapsulated hepatocytes were enhanced. Particularly, the mechanical stability of the microcapsules was dramatically strengthened. The new photo-crosslinkable tetra-copolymer formulation described in this article has opened a way to the development of hepatocyte microencapsulation technology for bioartifical liver assist device.
History
School
Aeronautical, Automotive, Chemical and Materials Engineering
Department
Chemical Engineering
Published in
Biomaterials
Volume
25
Issue
17
Pages
3531 - 3540
Citation
QUEK, C.H. ... et al, 2004. Photo-crosslinkable microcapsules formed by polyelectrolyte copolymer and modified collagen for rat hepatocyte encapsulation. Biomaterials, 25 (17), pp.3531-3540
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