posted on 2015-04-22, 10:25authored byAna I. Barbosa, Poonam Gehlot, Kalpita Sidapra, Alexander D. Edwards, Nuno Reis
We present a new, power-free and flexible detection system named MCFphone for portable colorimetric and fluorescence quantitative sandwich immunoassay detection of prostate specific antigen (PSA). The MCFphone is composed by a smartphone integrated with a magnifying lens, a simple light source and a miniaturised immunoassay platform, the Microcapillary Film (MCF). The excellent transparency and flat geometry of fluoropolymer MCF allowed quantitation of PSA in the range 0.9 to 60. ng/ml with<7% precision in 13. min using enzymatic amplification and a chromogenic substrate. The lower limit of detection was further improved from 0.4 to 0.08. ng/ml in whole blood samples with the use of a fluorescence substrate. The MCFphone has shown capable of performing rapid (13 to 22. min total assay time) colorimetric quantitative and highly sensitive fluorescence tests with good %Recovery, which represents a major step in the integration of a new generation of inexpensive and portable microfluidic devices with commercial immunoassay reagents and off-the-shelf smartphone technology.
Funding
This work was co-funded by Capillary Film Technology Ltd, Loughborough University and EPSRC [grant no. EP/L013983/1].
History
School
Aeronautical, Automotive, Chemical and Materials Engineering
Department
Chemical Engineering
Published in
Biosensors and Bioelectronics
Volume
70
Pages
5 - 14
Citation
BARBOSA, A. ... et al, 2015. Portable smartphone quantitation of prostate specific antigen (PSA) in a fluoropolymer microfluidic device. Biosensors and Bioelectronics, 70, pp.5-14.
This work is made available according to the conditions of the Creative Commons Attribution 4.0 International (CC BY 4.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/ by/4.0/
Publication date
2015
Notes
This article was published as Open Access by Elsevier B.V. under the CC BY license(http://creativecommons.org/licenses/by/4.0/).