File(s) under permanent embargo
Reason: This item is currently closed access.
Purification of human prothrombin from Nitschmann fraction III using DEAE membrane radial flow chromatography
journal contributionposted on 12.05.2017, 14:21 by Tao SunTao Sun, Ge Chen, Yiping Liu, Fengrong Bu, Meijuan Wen
A DEAE membrane radial column (4x5 cm) was used to purify human prothrombin from Nitschmann fraction III and performances of the column were investigated. Sample flow-rate of 20-30 ml/min (i.e., 24-36 bed volumes/h) and elution velocity up to 40 ml/min (i.e., 48 bed volumes/h) were obtained without compromising the separation efficiency. Its breakthrough capacity was one-third to half that of the commercial DEAE Sepharose Fast Flow media. It was concluded that the novel column is an attractive alternate to traditional axial column.
- Aeronautical, Automotive, Chemical and Materials Engineering
- Chemical Engineering