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Purification of human prothrombin from Nitschmann fraction III using DEAE membrane radial flow chromatography

journal contribution
posted on 12.05.2017, 14:21 authored by Tao SunTao Sun, Ge Chen, Yiping Liu, Fengrong Bu, Meijuan Wen
A DEAE membrane radial column (4x5 cm) was used to purify human prothrombin from Nitschmann fraction III and performances of the column were investigated. Sample flow-rate of 20-30 ml/min (i.e., 24-36 bed volumes/h) and elution velocity up to 40 ml/min (i.e., 48 bed volumes/h) were obtained without compromising the separation efficiency. Its breakthrough capacity was one-third to half that of the commercial DEAE Sepharose Fast Flow media. It was concluded that the novel column is an attractive alternate to traditional axial column.

History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Chemical Engineering

Published in

Journal of Chromatography B: Biomedical Sciences and Applications

Volume

742

Issue

1

Pages

109 - 114

Citation

SUN, T. ... et al, 2000. Purification of human prothrombin from Nitschmann fraction III using DEAE membrane radial flow chromatography. Journal of Chromatography B: Biomedical Sciences and Applications, 742 (1), pp.109-114

Version

NA (Not Applicable or Unknown)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2000

Notes

This paper is Closed Access.

ISSN

0378-4347

Language

en

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