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Purification of prothrombin in Nitschmann fraction III by membrane radial column ion-exchange liquid chromatograph
journal contributionposted on 12.05.2017, 15:00 by Tao SunTao Sun, Yiping Liu, Fengrong Bu, Ge Chen, Meijuan Wen
method is described for the purification of human prothrombin complex concentrate ( PCC) from Nitschmann fraction Ⅲ by membrane radial column ion2exchange chromatography , which allows large sample volumes to be processed at low operation pressure. The Nitschmann fraction Ⅲ(15 g) was mixed with 1 000 mL 0. 06 mol/ L Tris2HCl (pH 7. 5) . The centrifuged supernatant (10 000 r/ min ,15 min ,20 ℃) was applied onto a DEAE ion2exchange liquid chromatographic column (XK216 DEAE fast flow Sepharose and DEAE membrane radical column chromatography) with almost the same excellent separation efficiency. The parameters of sample flow rate , elution flow rate and sample capacity were optimized.
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