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Rapid and non-invasive method to determine toxic levels of alcohols and γ-hydroxylbutyric acid in saliva samples by gas chromatography-differential mobility spectrometry

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journal contribution
posted on 14.01.2016, 10:10 by L. Criado-García, Dorota Ruszkiewicz, Gary Eiceman, Paul Thomas
A polydimethylsilicone oral sampler was used to extract methanol, ethanol, ethylene glycol, 1,3-propandiol and y-hydroxybutyric acid from samples of human saliva obtained using a passive-drool approach. The extracted compounds were recovered by thermal desorption, isolated by gas chromatography and detected with differential mobility spectrometry, operating with a programmed dispersion field. Complex signal behaviours were also observed that were consistent with hitherto unobserved fragmentation behaviours in differential mobility spectrometry. These yielded high-mobility fragments obscured within the envelope of the water-based reactant ion peak. Further, compensation field maxima shifts were also observed attributable to transport gas modification phenomena. Nevertheless, the responses obtained indicated that in-vivo saliva sampling with thermal desorption gas chromatography may be used to provide a semi-quantitative diagnostic screen over the toxicity threshold concentration ranges of 100 mg.dm-3 to 3 g.dm-3. A candidate method suitable for use in low resource settings for the non-invasive screening of patients intoxicated by alcohols and volatile sedatives has been demonstrated.

History

School

  • Science

Department

  • Chemistry

Published in

Journal Breath Research

Citation

CRIADO-GARCIA, L. ...et al., 2016. Rapid and non-invasive method to determine toxic levels of alcohols and γ-hydroxylbutyric acid in saliva samples by gas chromatography-differential mobility spectrometry. Journal of Breath Research, 10, 017101.

Publisher

© Institute of Physics

Version

AM (Accepted Manuscript)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2016

Notes

This paper was accepted for publication in the journal Journal of Breath Research and the definitive published version is available at http://dx.doi.org/10.1088/1752-7155/10/1/017101.

ISSN

1752-7155

eISSN

1752-7163

Language

en