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Satellite cell response to concurrent resistance exercise and high-intensity interval training in sedentary, overweight/obese, middle-aged individuals

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posted on 04.12.2017, 14:20 by Jamie K. Pugh, Steve H. Faulkner, Mark Turner, Myra A. Nimmo
Purpose Sarcopenia can begin from the 4–5th decade of life and is exacerbated by obesity and inactivity. A combination of resistance exercise (RE) and endurance exercise is recommended to combat rising obesity and inactivity levels. However, work continues to elucidate whether interference in adaptive outcomes occur when RE and endurance exercise are performed concurrently. This study examined whether a single bout of concurrent RE and high-intensity interval training (HIIT) alters the satellite cell response following exercise compared to RE alone. Methods Eight sedentary, overweight/obese, middle-aged individuals performed RE only (8 × 8 leg extensions at 70% 1RM), or RE + HIIT (10 × 1 min at 90% HRmax on a cycle ergometer). Muscle biopsies were collected from the vastus lateralis before and 96 h after the RE component to determine muscle fiber type-specific total (Pax7+ cells) and active (MyoD+ cells) satellite cell number using immunofluorescence microscopy. Results Type-I-specific Pax7+(P = 0.001) cell number increased after both exercise trials. Type-I-specific MyoD+(P = 0.001) cell number increased after RE only. However, an elevated baseline value in RE + HIIT compared to RE (P = 0.046) was observed, with no differences between exercise trials at 96 h (P = 0.21). Type-II-specific Pax7+and MyoD+ cell number remained unchanged after both exercise trials (all P ≥ 0.13). Conclusion Combining a HIIT session after a single bout of RE does not interfere with the increase in type-I-specific total, and possibly active, satellite cell number, compared to RE only. Concurrent RE + HIIT may offer a time-efficient way to maximise the physiological benefits from a single bout of exercise in sedentary, overweight/obese, middleaged individuals.

Funding

The present work was in part funded by the Technogym, The Wellness Company and National Institute for Health Research (NIHR) Diet, Lifestyle and Physical Activity Biomedical Research Unit based at University Hospitals of Leicester and Loughborough University.

History

School

  • Sport, Exercise and Health Sciences

Published in

European Journal of Applied Physiology

Citation

PUGH, J.K. ...et al, 2018. Satellite cell response to concurrent resistance exercise and high-intensity interval training in sedentary, overweight/obese, middle-aged individuals. European Journal of Applied Physiology, 118 (2), pp. 225–238.

Publisher

© Springer

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution 4.0 Unported Licence (CC BY 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by/4.0/

Publication date

2017-10-25

Notes

This is an Open Access Article. It is published by Springer under the Creative Commons Attribution 4.0 Unported Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/

ISSN

1439-6319

eISSN

1439-6327

Language

en