Manuscript.pdf (802.77 kB)
Download file

Scale-down studies for assessing the impact of different stress parameters on growth and product quality during animal cell culture

Download (802.77 kB)
journal contribution
posted on 12.06.2014, 12:52 authored by Alvin W. Nienow, William H. Scott, Christopher Hewitt, Colin R. Thomas, Gareth Lewis, Ashraf Amanullah, Robert Kiss, Steven J. Meier
Two series of reproducible fed-batch bench scale cultures have been undertaken, one series simulating the impact of spatial variations in pH and nutrients as found at commercial scale on performance, the other, the impact of fluid dynamic stresses associated with agitation. The first was unsuccessful because, somewhat surprisingly, the use of a peristaltic pump to circulate cells and medium through different spatial environments always led to a similar reduction in culture time and resulting product titre compared to uncirculated controls. This fall was sufficient to essentially mask other effects. In the second, even at maximum specific energy dissipation rates up to ~160 times > with laminar extensional flow and ~25 times > with turbulent flow compared to typical commercial conditions, no significant effects were observed on cell growth and viability. Most importantly, in all of the cases studied, product quality was unaffected compared to controls. In addition, it is suggested that because of the possibility of cell line specific behaviour and the relationship between damage to entities and the Kolmogorov scale of turbulence, sensitivity to fluid dynamic stresses is best studied in turbulent bench scale bioreactors. © 2013 The Institution of Chemical Engineers.

History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Chemical Engineering

Published in

Chemical Engineering Research and Design

Volume

91

Issue

11

Pages

2265 - 2274

Citation

NIENOW, A.W. ... et al, 2013. Scale-down studies for assessing the impact of different stress parameters on growth and product quality during animal cell culture. Chemical Engineering Research and Design, 91 (11), pp. 2265 - 2274

Publisher

Elsevier / © The Institution of Chemical Engineers

Version

AM (Accepted Manuscript)

Publication date

2013

Notes

This is the author’s version of a work that was accepted for publication in Chemical Engineering Research and Design. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Chemical Engineering Research and Design, 91 (11), pp. 2265 - 2274, DOI: 10.1016/j.cherd.2013.04.002

ISSN

0263-8762

Language

en