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Self-organization of skin cells in three-dimensional electrospun polystyrene scaffolds

journal contribution
posted on 12.05.2017, 13:36 by Tao SunTao Sun, Shaoming Mai, David Norton, John W. Haycock, Anthony J. Ryan, Sheila MacNeil
Much research in tissue engineering focuses on the synthesis of complex three-dimensional polymer scaffolds containing functional biomolecules to which cells are introduced. Typical scaffolds for skin tissue engineering are macroscopically porous with struts or fibers 10 m thick at a packing fraction of 0.1. We made a polystyrene scaffold without cell signaling or spatial information by electrospinning and studied the growth of skin fibroblasts, keratinocytes, and endothelial cells, as single and cocultured populations in the presence and absence of fetal calf serum. In the absence of serum, keratinocytes, fibroblasts, and endothelial cells did not grow when cultured alone. However, when fibroblasts were cocultured with keratinocytes and endothelial cells, expansion of keratinocytes and endothelial cells occurred even in the absence of serum. Furthermore, cells displayed native spatial three-dimensional organization when cultured at an air–liquid interface, even when all three cell types were introduced at random to the scaffold. This study shows that coculture with fibroblasts enables keratinocytes and endothelial cells to proliferate without serum, but also to self-organize according to the native epidermal–dermal structure given the symmetry-breaking field of an air–liquid interface.

History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Chemical Engineering

Published in

Tissue Engineering

Volume

11

Issue

7-8

Pages

1023 - 1033

Citation

SUN, T. ... et al, 2005. Self-organization of skin cells in three-dimensional electrospun polystyrene scaffolds. Tissue Engineering, 11 (7-8), pp. 1023-1033

Publisher

© Mary Ann Liebert, Inc.

Version

NA (Not Applicable or Unknown)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2005

Notes

This paper is closed access.

ISSN

1076-3279

eISSN

1557-8690

Language

en

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