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Short-term high-fat overfeeding does not induce NF-κB inflammatory signaling in subcutaneous white adipose tissue

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journal contribution
posted on 02.04.2020, 12:59 by Rebecca Dewhurst-Trigg, Alex J Wadley, Rachel Woods, Lauren SherarLauren Sherar, Nicolette BishopNicolette Bishop, Carl Hulston, Oonagh MarkeyOonagh Markey
Context
It is unclear how white adipose tissue (WAT) inflammatory signaling proteins respond during the early stages of overnutrition.
Objective
To investigate the effect of short-term, high-fat overfeeding on fasting abdominal subcutaneous WAT total content and phosphorylation of proteins involved in nuclear factor-κB (NF-κB) inflammatory signaling, systemic metabolic measures and inflammatory biomarkers.
Design
Individuals consumed a high-fat (65% total energy total fat), high-energy (50% above estimated energy requirements) diet for 7 days.
Results Fifteen participants (age 27 ± 1 y; BMI 24.4 ± 0.6 kg/m2) completed the study. Body mass increased following high-fat overfeeding (+1.2 ± 0.2 kg; P < 0.0001). However, total content and phosphorylation of proteins involved in NF-κB inflammatory signaling were unchanged following the intervention. Fasting serum glucose (+0.2 ± 0.0 mmol/L), total cholesterol (+0.4 ± 0.1 mmol/L), low-density lipoprotein cholesterol (+0.3 ± 0.1 mmol/L), high-density lipoprotein cholesterol (+0.2 ± 0.0 mmol/L), and lipopolysaccharide-binding protein (LBP; +4.7 ± 2.1 µg/mL) increased, whereas triacylglycerol concentrations (-0.2 ± 0.1 mmol/L) decreased following overfeeding (all P < 0.05). Systemic biomarkers (insulin, soluble cluster of differentiation 14 (CD14), C-reactive protein, IL-6, TNF-α and monocyte chemoattractant protein-1) and the proportion and concentration of circulating CD14+ monocytes were unaffected by overfeeding.
Conclusion
Acute lipid oversupply did not impact on total content or phosphorylation of proteins involved in WAT NF-κB inflammatory signaling, despite modest weight gain and metabolic alterations. Systemic LBP, which is implicated in the progression of low-grade inflammation during the development of obesity, increased in response to a 7-day high-fat overfeeding period.

Funding

This research was supported by an Early Career Grant from the Society for Endocrinology (UK) (to OM) and co-funded by the National Institute for Health Research (NIHR) Leicester Biomedical Research Centre.

History

School

  • Sport, Exercise and Health Sciences

Published in

The Journal of Clinical Endocrinology & Metabolism

Volume

105

Issue

7

Pages

2162 - 2176

Publisher

Oxford University Press and The Endocrine Society

Version

AM (Accepted Manuscript)

Rights holder

© Endocrine Society

Publisher statement

This is a pre-copyedited, author-produced version of an article accepted for publication in The Journal of Clinical Endocrinology & Metabolism following peer review. The version of record Rebecca Dewhurst-Trigg, Alex J Wadley, Rachel M Woods, Lauren B Sherar, Nicolette C Bishop, Carl J Hulston, Oonagh Markey, Short-term high-fat overfeeding does not induce NF-κB inflammatory signaling in subcutaneous white adipose tissue, The Journal of Clinical Endocrinology & Metabolism, 105 (7), pp.2162-2176, https://doi.org/10.1210/clinem/dgaa158 is available online at: https://academic.oup.com/jcem/article/105/7/2162/5813979 and https://doi.org/10.1210/clinem/dgaa158.

Acceptance date

27/03/2020

Publication date

2020-03-31

Copyright date

2020

ISSN

0021-972X

eISSN

1945-7197

Language

en

Depositor

Dr Oonagh Markey. Deposit date: 1 April 2020