Dewhurst-Trigg et al._2020_JCEM_Accepted version (1).pdf (1018.81 kB)
Download fileShort-term high-fat overfeeding does not induce NF-κB inflammatory signaling in subcutaneous white adipose tissue
journal contribution
posted on 2020-04-02, 12:59 authored by Rebecca Dewhurst-Trigg, Alex J Wadley, Rachel Woods, Lauren SherarLauren Sherar, Nicolette BishopNicolette Bishop, Carl Hulston, Oonagh MarkeyOonagh MarkeyContext
It is unclear how white adipose tissue (WAT) inflammatory signaling proteins respond during the early stages of overnutrition.
Objective
To investigate the effect of short-term, high-fat overfeeding on fasting abdominal subcutaneous WAT total content and phosphorylation of proteins involved in nuclear factor-κB (NF-κB) inflammatory signaling, systemic metabolic measures and inflammatory biomarkers.
Design
Individuals consumed a high-fat (65% total energy total fat), high-energy (50% above estimated energy requirements) diet for 7 days.
Results
Fifteen participants (age 27 ± 1 y; BMI 24.4 ± 0.6 kg/m2) completed the study. Body mass increased following high-fat overfeeding (+1.2 ± 0.2 kg; P < 0.0001). However, total content and phosphorylation of proteins involved in NF-κB inflammatory signaling were unchanged following the intervention. Fasting serum glucose (+0.2 ± 0.0 mmol/L), total cholesterol (+0.4 ± 0.1 mmol/L), low-density lipoprotein cholesterol (+0.3 ± 0.1 mmol/L), high-density lipoprotein cholesterol (+0.2 ± 0.0 mmol/L), and lipopolysaccharide-binding protein (LBP; +4.7 ± 2.1 µg/mL) increased, whereas triacylglycerol concentrations (-0.2 ± 0.1 mmol/L) decreased following overfeeding (all P < 0.05). Systemic biomarkers (insulin, soluble cluster of differentiation 14 (CD14), C-reactive protein, IL-6, TNF-α and monocyte chemoattractant protein-1) and the proportion and concentration of circulating CD14+ monocytes were unaffected by overfeeding.
Conclusion
Acute lipid oversupply did not impact on total content or phosphorylation of proteins involved in WAT NF-κB inflammatory signaling, despite modest weight gain and metabolic alterations. Systemic LBP, which is implicated in the progression of low-grade inflammation during the development of obesity, increased in response to a 7-day high-fat overfeeding period.
Funding
This research was supported by an Early Career Grant from the Society for Endocrinology (UK) (to OM) and co-funded by the National Institute for Health Research (NIHR) Leicester Biomedical Research Centre.
History
School
- Sport, Exercise and Health Sciences
Published in
The Journal of Clinical Endocrinology & MetabolismVolume
105Issue
7Pages
2162 - 2176Publisher
Oxford University Press and The Endocrine SocietyVersion
- AM (Accepted Manuscript)
Rights holder
© Endocrine SocietyPublisher statement
This is a pre-copyedited, author-produced version of an article accepted for publication in The Journal of Clinical Endocrinology & Metabolism following peer review. The version of record Rebecca Dewhurst-Trigg, Alex J Wadley, Rachel M Woods, Lauren B Sherar, Nicolette C Bishop, Carl J Hulston, Oonagh Markey, Short-term high-fat overfeeding does not induce NF-κB inflammatory signaling in subcutaneous white adipose tissue, The Journal of Clinical Endocrinology & Metabolism, 105 (7), pp.2162-2176, https://doi.org/10.1210/clinem/dgaa158 is available online at: https://academic.oup.com/jcem/article/105/7/2162/5813979 and https://doi.org/10.1210/clinem/dgaa158.Acceptance date
2020-03-27Publication date
2020-03-31Copyright date
2020ISSN
0021-972XeISSN
1945-7197Publisher version
Language
- en