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Simultaneous determination of the mechanical properties and turgor of a single bacterial cell using atomic force microscopy

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posted on 2024-02-12, 16:51 authored by Rui Han, Waldemar Vollmer, John D Perry, Paul Stoodley, Jinju ChenJinju Chen

Bacterial mechanical properties (cell wall stiffness and turgor) are important factors for bacterial survival in harsh environments. For an individual bacterial cell, it is challenging to determine the cell wall stiffness and turgor simultaneously. In this study, we adopted a combined finite element modelling and mathematical modelling approach to simultaneously determine bacterial cell wall stiffness and turgor of an individual bacterial cell based on atomic force microscopy (AFM) nanoindentation. The mechanical properties and turgor of Staphylococcus epidermidis, determined by our method are consistent with other independent studies. For a given aqueous environment, bacterial cell wall stiffness increased linearly with an increase in turgor. Higher osmolarity leads to a decrease in both cell wall stiffness and turgor. We also demonstrated that the change of turgor is associated with a change in viscosity of the bacterial cell.

Funding

Multiscale characterization of complex materials using a combination of atomic force microscopy and optical coherence tomography

Engineering and Physical Sciences Research Council

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Biofilm Resistant Liquid-like Solid Surfaces in Flow Situations

Engineering and Physical Sciences Research Council

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The Physics of Antimicrobial Resistance

Engineering and Physical Sciences Research Council

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History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Materials

Published in

Nanoscale

Volume

14

Issue

33

Pages

12060 - 12068

Publisher

Royal Society of Chemistry

Version

  • VoR (Version of Record)

Rights holder

© The Authors

Publisher statement

This is an Open Access Article. It is published by the Royal Society of Chemistry under the Creative Commons Attribution 3.0 Unported Licence (CC BY). Full details of this licence are available at: https://creativecommons.org/licenses/by/3.0/

Acceptance date

2022-08-06

Publication date

2022-08-08

Copyright date

2022

ISSN

2040-3364

eISSN

2040-3372

Language

  • en

Depositor

Prof Jinju Chen. Deposit date: 11 February 2024

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