Gadolinium paper - final version.pdf (389.3 kB)
Single cell tracking of gadolinium labeled CD4(+) T cells by laser ablation inductively coupled plasma mass spectrometry
journal contribution
posted on 2014-10-14, 15:41 authored by Amy ManaghAmy Managh, Sheldon L. Edwards, Andrew Bushell, Kathryn J. Wood, Edward K. Geissler, James A. Hutchinson, Robert W. Hutchinson, Helen Reid, Barry SharpCellular therapy is emerging as a promising alternative to conventional immunosuppression in the fields of haematopoietic stem cell (HSC) transplantation, autoimmune disease and solid organ transplantation. Determining the persistence of cell-based therapies in vivo is crucial to understanding their regulatory function and requires the combination of an extremely sensitive detection technique and a stable, long-lifetime cell labelling agent. This paper reports the first application of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) to perform single cell detection of T cell populations relevant to cellular immunotherapy. Purified human CD4+ T cells were labelled with commercially available Gd-based MRI contrast agents, Omniscan® and Dotarem®, which enabled passive loading of up to 108 Gd atoms per cell. In mixed preparations of labelled and unlabelled cells, LA-ICP-MS was capable of enumerating labelled cells at close to the predicted ratio. More importantly, LA-ICP-MS single cell analysis demonstrated that the cells retained sufficient label to remain detectable for up to 10 days post-labelling both in vitro and in vivo in an immunodeficient mouse model.
Funding
Support received from the ONE Study, a European Commission Seventh Framework Program funded project (EU FP7-HEALTH “The ONE Study”, Project reference 260687), and the British Heart Foundation PG 10/62/28504 is gratefully acknowledged.
History
School
- Science
Department
- Chemistry
Published in
ANALYTICAL CHEMISTRYVolume
85Issue
22Pages
10627 - 10634 (8)Citation
MANAGH, A.J. ... et al, 2013. Single cell tracking of gadolinium labeled CD4(+) T cells by laser ablation inductively coupled plasma mass spectrometry. Analytical Chemistry, 85 (22), pp.10627-10634.Publisher
© American Chemical SocietyVersion
- AM (Accepted Manuscript)
Publication date
2013Notes
This document is the Accepted Manuscript version of a Published Work that appeared in final form in [Analytical Chemistry], copyright © American Chemical Society after peer review and technical editing by the publisher. The final edited and published work can be found at: http://dx.doi.org/10.1021/ac4022715ISSN
0003-2700Publisher version
Language
- en