Gadolinium paper - final version.pdf (389.3 kB)
Download file

Single cell tracking of gadolinium labeled CD4(+) T cells by laser ablation inductively coupled plasma mass spectrometry

Download (389.3 kB)
journal contribution
posted on 14.10.2014, 15:41 authored by Amy ManaghAmy Managh, Sheldon L. Edwards, Andrew Bushell, Kathryn J. Wood, Edward K. Geissler, James A. Hutchinson, Robert W. Hutchinson, Helen Reid, Barry Sharp
Cellular therapy is emerging as a promising alternative to conventional immunosuppression in the fields of haematopoietic stem cell (HSC) transplantation, autoimmune disease and solid organ transplantation. Determining the persistence of cell-based therapies in vivo is crucial to understanding their regulatory function and requires the combination of an extremely sensitive detection technique and a stable, long-lifetime cell labelling agent. This paper reports the first application of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) to perform single cell detection of T cell populations relevant to cellular immunotherapy. Purified human CD4+ T cells were labelled with commercially available Gd-based MRI contrast agents, Omniscan® and Dotarem®, which enabled passive loading of up to 108 Gd atoms per cell. In mixed preparations of labelled and unlabelled cells, LA-ICP-MS was capable of enumerating labelled cells at close to the predicted ratio. More importantly, LA-ICP-MS single cell analysis demonstrated that the cells retained sufficient label to remain detectable for up to 10 days post-labelling both in vitro and in vivo in an immunodeficient mouse model.

Funding

Support received from the ONE Study, a European Commission Seventh Framework Program funded project (EU FP7-HEALTH “The ONE Study”, Project reference 260687), and the British Heart Foundation PG 10/62/28504 is gratefully acknowledged.

History

School

  • Science

Department

  • Chemistry

Published in

ANALYTICAL CHEMISTRY

Volume

85

Issue

22

Pages

10627 - 10634 (8)

Citation

MANAGH, A.J. ... et al, 2013. Single cell tracking of gadolinium labeled CD4(+) T cells by laser ablation inductively coupled plasma mass spectrometry. Analytical Chemistry, 85 (22), pp.10627-10634.

Publisher

© American Chemical Society

Version

AM (Accepted Manuscript)

Publication date

2013

Notes

This document is the Accepted Manuscript version of a Published Work that appeared in final form in [Analytical Chemistry], copyright © American Chemical Society after peer review and technical editing by the publisher. The final edited and published work can be found at: http://dx.doi.org/10.1021/ac4022715

ISSN

0003-2700

Language

en

Usage metrics

Keywords

Exports