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Single cell tracking of gadolinium labeled CD4(+) T cells by laser ablation inductively coupled plasma mass spectrometry

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journal contribution
posted on 14.10.2014, 15:41 by Amy ManaghAmy Managh, Sheldon L. Edwards, Andrew Bushell, Kathryn J. Wood, Edward K. Geissler, James A. Hutchinson, Robert W. Hutchinson, Helen Reid, Barry Sharp
Cellular therapy is emerging as a promising alternative to conventional immunosuppression in the fields of haematopoietic stem cell (HSC) transplantation, autoimmune disease and solid organ transplantation. Determining the persistence of cell-based therapies in vivo is crucial to understanding their regulatory function and requires the combination of an extremely sensitive detection technique and a stable, long-lifetime cell labelling agent. This paper reports the first application of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) to perform single cell detection of T cell populations relevant to cellular immunotherapy. Purified human CD4+ T cells were labelled with commercially available Gd-based MRI contrast agents, Omniscan® and Dotarem®, which enabled passive loading of up to 108 Gd atoms per cell. In mixed preparations of labelled and unlabelled cells, LA-ICP-MS was capable of enumerating labelled cells at close to the predicted ratio. More importantly, LA-ICP-MS single cell analysis demonstrated that the cells retained sufficient label to remain detectable for up to 10 days post-labelling both in vitro and in vivo in an immunodeficient mouse model.

Funding

Support received from the ONE Study, a European Commission Seventh Framework Program funded project (EU FP7-HEALTH “The ONE Study”, Project reference 260687), and the British Heart Foundation PG 10/62/28504 is gratefully acknowledged.

History

School

  • Science

Department

  • Chemistry

Published in

ANALYTICAL CHEMISTRY

Volume

85

Issue

22

Pages

10627 - 10634 (8)

Citation

MANAGH, A.J. ... et al, 2013. Single cell tracking of gadolinium labeled CD4(+) T cells by laser ablation inductively coupled plasma mass spectrometry. Analytical Chemistry, 85 (22), pp.10627-10634.

Publisher

© American Chemical Society

Version

AM (Accepted Manuscript)

Publication date

2013

Notes

This document is the Accepted Manuscript version of a Published Work that appeared in final form in [Analytical Chemistry], copyright © American Chemical Society after peer review and technical editing by the publisher. The final edited and published work can be found at: http://dx.doi.org/10.1021/ac4022715

ISSN

0003-2700

Language

en

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