Single cell tracking of gadolinium labeled CD4(+) T cells by laser ablation inductively coupled plasma mass spectrometry
journal contributionposted on 14.10.2014, 15:41 authored by Amy ManaghAmy Managh, Sheldon L. Edwards, Andrew Bushell, Kathryn J. Wood, Edward K. Geissler, James A. Hutchinson, Robert W. Hutchinson, Helen Reid, Barry Sharp
Cellular therapy is emerging as a promising alternative to conventional immunosuppression in the fields of haematopoietic stem cell (HSC) transplantation, autoimmune disease and solid organ transplantation. Determining the persistence of cell-based therapies in vivo is crucial to understanding their regulatory function and requires the combination of an extremely sensitive detection technique and a stable, long-lifetime cell labelling agent. This paper reports the first application of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) to perform single cell detection of T cell populations relevant to cellular immunotherapy. Purified human CD4+ T cells were labelled with commercially available Gd-based MRI contrast agents, Omniscan® and Dotarem®, which enabled passive loading of up to 108 Gd atoms per cell. In mixed preparations of labelled and unlabelled cells, LA-ICP-MS was capable of enumerating labelled cells at close to the predicted ratio. More importantly, LA-ICP-MS single cell analysis demonstrated that the cells retained sufficient label to remain detectable for up to 10 days post-labelling both in vitro and in vivo in an immunodeficient mouse model.
Support received from the ONE Study, a European Commission Seventh Framework Program funded project (EU FP7-HEALTH “The ONE Study”, Project reference 260687), and the British Heart Foundation PG 10/62/28504 is gratefully acknowledged.