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Download fileStem cell delivery with polymer hydrogel for treatment of intervertebral disc degeneration: from 3D culture to design of the delivery device for minimally invasive therapy
journal contribution
posted on 2016-08-03, 13:23 authored by Deepak Kumar, Alex Lyness, Irini Gerges, Cristina Lenardi, Nicholas R. Forsyth, Yang LiuYang LiuNucleus pulposus (NP) tissue damage can induce detrimental mechanical strain on the
biomechanical performance of intervertebral disc (IVD) causing subsequent disc
degeneration. A novel, photocurable, injectable, synthetic polymer hydrogel
(pHEMA-co-APMA grafted with PAA) has already demonstrated success in encapsulating and differentiating human mesenchymal stem cells (hMSCs) towards an NP phenotype during hypoxic conditions. After demonstration of promising results in our previous work, in this study, we have further investigated the inclusion of mechanical stimulation and its impact on hMSC differentiation towards an NP
phenotype through the characterization of matrix markers such as SOX-9, Aggrecan
and Collagen II. Furthermore, investigations were undertaken in order to approximate
delivery parameters for an injection delivery device, which could be used to transport hMSCs suspended in hydrogel into the IVD. hMSC laden hydrogel solutions were
injected through various needle gauge sizes in order to deter its impact on postinjection
cell viability and IVD tissue penetration. Interpretation of this data informed the design of a potential minimally invasive injection device, which could successfully inject hMSCs encapsulated in a UV curable polymer into NP, prior to photocrosslinking in situ.
Funding
This study was supported by the EPSRC Centre of Innovative Manufacturing in Regenerative Medicine, Royal Society funding for International Joint Project and FP7 SkelGen.
History
School
- Mechanical, Electrical and Manufacturing Engineering
Published in
Cell TransplantationVolume
25Issue
12Pages
2213-2220Citation
KUMAR, D. ...et al., 2016. Stem cell delivery with polymer hydrogel for treatment of intervertebral disc degeneration: from 3D culture to design of the delivery device for minimally invasive therapy. Cell Transplantation, 25 (12), pp. 2213-2220.Publisher
Cognizant Communication CorporationVersion
- VoR (Version of Record)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution 3.0 Unported (CC BY 3.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/by/3.0/Acceptance date
2016-07-13Publication date
2016-12-01Copyright date
2016Notes
This is an Open Access Article. It is published by Cognizant Communication Corporation under the Creative Commons Attribution 3.0 Unported Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/3.0/ISSN
1555-3892Publisher version
Language
- en