Loughborough University
stiffness-of-cell-microenvironment-guides-long-term-cell-growth-in-cell-seeded-collagen-microspheres.pdf (1.31 MB)

Stiffness of cell micro-environment guides long term cell growth in cell seeded collagen microspheres

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journal contribution
posted on 2018-10-16, 10:26 authored by Maryam Shariatzadeh, C.M. Perrault, D. Lacroix
Mesenchymal stem cells are widely implicated as a cell source for tissue engineering of skeletal tissue in cell-based therapy. Physical and mechanical cues are potent controlling factors in cell differentiation and can be implemented as a guide to study cellular response, matrix production and tissue regeneration. Microspheres were produced by gelation of bovine collagen type I with concentration of 2 mg/mL and 1,000-2,000 cells per droplet. Short and long term cell viability of human embryonic stem cell-derived mesenchymal progenitors (hES-MPs) and MG-63 osteoblastic cells as well as collagen microstructure and contraction were monitored during 28 days post encapsulation (pc). Results indicated that collagen concentration, hence mechanical properties of cell’s extracellular micro-environment are important in cell proliferation and differentiation. Contraction of cell-embedded microspheres was found to be vital in cell adaptation and the remodelling of their new environment. It was also found that collagen concentration of 2 mg/mL supports proliferation of hES-MPs while higher collagen concentration promoted the viability of MG-63s. Results of hES-MPs characterization in 3D soft environment and mechanically stimulated hES-MPs collagen microspheres can be used in cells/therapeutic carriers, implants in bone and cartilage healing applications. The microspheres developed in this study can also be used as a tool to build more optimised construct to transfer mechanically stimulated stem cells to the specific area of a defective bone which would add significant benefit to the field of bone regeneration.


Financial support from the European Research Council (258321) is acknowledged.



  • Mechanical, Electrical and Manufacturing Engineering

Published in

Archives of Clinical and Biomedical Research






167 - 182


SHARIATZADEH, M., PERRAULT, C.M. and LACROIX, D., 2018. Stiffness of cell micro-environment guides long term cell growth in cell seeded collagen microspheres. Archives of Clinical and Biomedical Research, 2 (5), pp.167-182.


Fortune Journals


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This is an Open Access Article. It is published by Fortune Journals under the Creative Commons Attribution 4.0 International Licence (CC BY). Full details of this licence are available at: https://creativecommons.org/licenses/by/4.0/




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