Clifford et al. 2017 Tregs.pdf (2.39 MB)
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T-regulatory cells exhibit a biphasic response to prolonged endurance exercise in humans

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journal contribution
posted on 21.10.2019, 09:22 authored by Tom CliffordTom Clifford, Matthew J Wood, Philip Stocks, Glyn Howatson, Emma J Stevenson, Catharien MU Hilkens
Purpose: T-regulatory cells (Tregs) are a sub-population of lymphocytes that act to suppress aberrant immune responses. We investigated changes in the numbers of naïve and terminally differentiated Tregs in the peripheral blood to establish their role in the immuno-suppressive response to prolonged exercise. Methods: Blood was drawn from seventeen experienced runners (age 40 ± 12 years; height 1.75 ± 0.08 m; mass 71.4 ± 10.8 kg) before, ~1 h after (POST-1h), and on the day following the marathon (POST-1d). Tregs (CD3+CD4+Foxp3+CD25++CD127−) were analysed in peripheral blood mononuclear cells using flow cytometry. The markers CD45RA and HLA-DR were included to define naïve and terminally differentiated Tregs, respectively. Results: The absolute number of Tregs decreased (27%) POST-1h marathon (P < 0.001) but increased (21%) at POST-1d (P < 0.01). Naïve CD45RA+ Tregs fell by 39% POST-1h (P < 0.01) but were unaffected POST-1d (P > 0.05). In contrast, an increased number of Tregs expressing HLA-DR was observed at POST-1d (P < 0.01). Interleukin (IL)-1β, IL-6, IL-8 and IL-10 levels in the serum all increased POST-1h (P > 0.05) but returned to pre-exercise levels POST-1d. The suppressive cytokine, transforming growth factor-beta, was unaffected by the marathon (P > 0.05). Conclusions: These results suggest that Tregs do not play a major role in immune suppression in the early hours of recovery from a marathon. However, terminally differentiated HLA-DR+ Tregs are mobilized the following day, which could represent a compensatory attempt by the host to restore immune homeostasis and limit excessive cell damage.

History

School

  • Sport, Exercise and Health Sciences

Published in

European Journal of Applied Physiology

Volume

117

Issue

8

Pages

1727 - 1737

Publisher

Springer

Version

VoR (Version of Record)

Rights holder

© The Authors

Acceptance date

17/06/2017

Publication date

2017-06-23

Copyright date

2017

ISSN

1439-6319

eISSN

1439-6327

Language

en

Depositor

Dr Tom Clifford. Deposit date: 18 October 2019

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