Ferguson et al BFR angiogenic signalling - EJSS accepted.pdf (386.13 kB)
The acute angiogenic signalling response to low-load resistance exercise with blood flow restriction
journal contribution
posted on 2018-02-01, 14:23 authored by Richard FergusonRichard Ferguson, Julie E.A. Hunt, Mark LewisMark Lewis, Neil MartinNeil Martin, Darren J. Player, Carolin Stangier, Conor W. Taylor, Mark TurnerThis study investigated protein kinase activation and gene expression of angiogenic factors in response to low-load resistance exercise with or without blood flow restriction (BFR). In a repeated measures cross-over design, six males performed four sets of bilateral knee extension exercise at 20% 1RM (reps per set = 30:15:15:continued to fatigue) with BFR (110 mmHg) and without (CON). Muscle biopsies were obtained from the vastus lateralis before, 2 and 4 h post-exercise. mRNA expression was determined using real-time RT-PCR. Protein phosphorylation/expression was determined using Western blot. p38MAPK phosphorylation was greater (p = 0.05) at 2 h following BFR (1.3 ± 0.8) compared to CON (0.4 ± 0.3). AMPK phosphorylation remained unchanged. PGC-1α mRNA expression increased at 2 h (5.9 ± 1.3 vs. 2.1 ± 0.8; p = 0.03) and 4 h (3.2 ± 0.8 vs. 1.5 ± 0.4; p = 0.03) following BFR exercise with no change in CON. PGC-1α protein expression did not change following either exercise. BFR exercise enhanced mRNA expression of vascular endothelial growth factor (VEGF) at 2 h (5.2 ± 2.8 vs 1.7 ± 1.1; p = .02) and 4 h (6.8 ± 4.9 vs. 2.5 ± 2.7; p = .01) compared to CON. mRNA expression of VEGF-R2 and hypoxia-inducible factor 1α increased following BFR exercise but only eNOS were enhanced relative to CON. Matrix metalloproteinase-9 mRNA expression was not altered in response to either exercise. Acute low-load resistance exercise with BFR provides a targeted angiogenic response potentially mediated through enhanced ischaemic and shear stress stimuli.
History
School
- Sport, Exercise and Health Sciences
Published in
Eur J Sport SciPages
1 - 10Citation
FERGUSON, R.A. ... et al., 2018. The acute angiogenic signalling response to low-load resistance exercise with blood flow restriction. European Journal of Sport Science, 18 (3), pp.397-406.Publisher
© European College of Sport Science. Published by Taylor and FrancisVersion
- AM (Accepted Manuscript)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Acceptance date
2017-12-18Publication date
2018-01-17Notes
This is an Accepted Manuscript of an article published by Taylor & Francis in European Journal of Sport Science on 17 January 2018, available online: http://www.tandfonline.com/10.1080/17461391.2017.1422281.ISSN
1746-1391eISSN
1536-7290Publisher version
Language
- en