Shift reagents in Ion Mobility Spectrometry_J Am Mass Spectrom 2009.pdf (445.42 kB)
The use of shift reagents in ion mobility-mass spectrometry: studies on the complexation of an active pharmaceutical ingredient with polyethylene glycol excipients
journal contributionposted on 2009-03-25, 16:42 authored by Mark D. Howdle, Christine Eckers, Alice M.-F. Laures, Colin Creaser
Gas-phase ion mobility studies of mixtures containing polyethylene glycols (PEG) and an active pharmaceutical ingredient (API), Lamivudine, have been carried out using electrospray ionization-ion mobility spectrometry-quadrupole-time-of-flight mass spectrometry (ESI-IMS-Q-TOF). In addition to protonated and cationised PEG oligomers, a series of high molecular weight ions were observed and identified as non-covalent complexes formed between Lamivudine and PEG oligomers. The non-covalent complex ions were dissociated using collision induced dissociation (CID) after separation in the ion mobility drift tube to recover the protonated Lamivudine free from interfering matrix ions and with a drift time associated with the precursor complex. The potential of PEG excipients to act as ‘shift reagents’, which enhance selectivity by moving the mass/mobility locus to an area of the spectrum away from interferences, is demonstrated for the analysis of Lamivudine in a Combivir formulation containing PEG and Lamivudine.
CitationHOWDLE, M. ... et al, 2009. The use of shift reagents in ion mobility-mass spectrometry: studies on the complexation of an active pharmaceutical ingredient with polyethylene glycol excipients. Journal of the American Society for Mass Spectrometry, 20 (1), pp.1-9
PublisherElsevier / © American Society for Mass Spectrometry
- AM (Accepted Manuscript)
NotesThis article was published in the journal, Journal of the American Society for Mass Spectrometry [© American Society for Mass Spectrometry] and the definitive version is available at: http://dx.doi.org/10.1016/j.jasms.2008.10.002