Shift reagents in Ion Mobility Spectrometry_J Am Mass Spectrom 2009.pdf (445.42 kB)
Download fileThe use of shift reagents in ion mobility-mass spectrometry: studies on the complexation of an active pharmaceutical ingredient with polyethylene glycol excipients
journal contribution
posted on 2009-03-25, 16:42 authored by Mark D. Howdle, Christine Eckers, Alice M.-F. Laures, Colin CreaserGas-phase ion mobility studies of mixtures containing polyethylene glycols (PEG) and an active pharmaceutical ingredient (API), Lamivudine, have been carried out using electrospray ionization-ion mobility spectrometry-quadrupole-time-of-flight mass spectrometry (ESI-IMS-Q-TOF). In addition to protonated and cationised PEG oligomers, a series of high molecular weight ions were observed and identified as non-covalent complexes formed between Lamivudine and PEG oligomers. The non-covalent complex ions were dissociated using collision induced dissociation (CID) after separation in the ion mobility drift tube to recover the protonated Lamivudine free from interfering matrix ions and with a drift time associated with the precursor complex. The potential of PEG excipients to act as ‘shift reagents’, which enhance selectivity by moving the mass/mobility locus to an area of the spectrum away from interferences, is demonstrated for the analysis of Lamivudine in a Combivir formulation containing PEG and Lamivudine.
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