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Transmembrane transport of bicarbonate unravelled

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journal contribution
posted on 05.05.2021, 13:17 authored by Luis Martínez‐Crespo, Sarah Hewitt, Nicola Alessandro De Simone, Vladimír Šindelář, Anthony P Davis, Stephen ButlerStephen Butler, Hennie Valkenier
Anion receptors can be used to transport ions across lipid bilayers, which has potential for therapeutic applications. Synthetic bicarbonate transporters are of particular interest, as defects in transmembrane transport of bicarbonate are associated with various diseases. However, no convenient method exists to directly observe bicarbonate transport and study the mechanisms involved. Here, an assay is presented that allows the kinetics of bicarbonate transport into liposomes to be monitored directly and with great sensitivity. The assay utilises an encapsulated europium(III) complex, which exhibits a large increase in emission intensity upon binding bicarbonate. Mechanisms involving CO2 diffusion and the dissipation of a pH gradient are shown to be able to lead to an increase in bicarbonate concentration within liposomes, without transport of the anion occurring at all. By distinguishing these alternative mechanisms from actual bicarbonate transport, this assay will inform the future development of bicarbonate transporters.

Funding

European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (Grant agreement No. 802727)

Luminescent Host Molecules for Multisite Recognition of Polyphosphate Anions

Engineering and Physical Sciences Research Council

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Wellcome Trust (204500/Z/16/Z)

Czech Science Foundation (No. 20-13922S)

History

School

  • Science

Department

  • Chemistry

Published in

Chemistry – A European Journal

Volume

27

Issue

26

Pages

7367-7375

Publisher

Wiley

Version

VoR (Version of Record)

Rights holder

© The Authors

Publisher statement

This is an Open Access Article. It is published by Wiley under the Creative Commons Attribution 4.0 International Licence (CC BY 4.0). Full details of this licence are available at: https://creativecommons.org/licenses/by/4.0/

Publication date

2021-05-01

Copyright date

2021

ISSN

0947-6539

eISSN

1521-3765

Language

en

Depositor

Dr Stephen Butler. Deposit date: 5 May 2021

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