Tuning colourimetric indicator displacement assays for naked-eye sensing of pyrophosphate in aqueous media

Liu, Xuejian; Ngo, Huy Tien; Ge, Zijun; Butler, Stephen; Jolliffe, Katrina A.

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Tuning colourimetric indicator displacement assays for naked-eye sensing of pyrophosphate in aqueous media

journal contribution

posted on 2015-09-15, 15:04 authored by Xuejian Liu, Huy Tien Ngo, Zijun Ge, Stephen ButlerStephen Butler, Katrina A. Jolliffe

A library of anion receptors comprising cyclic peptide scaffolds bearing two (ZnII-DPA) anion binding sites have been synthesised and their anion binding abilities evaluated using colourimetric indicator displacement assays with three different indicators. The resulting chemosensing ensembles provided excellent discrimination between polyphosphate ions, with several of the receptor:indicator combinations providing naked-eye sensing of pyrophosphate ions in aqueous solutions containing more than 100 fold excess of ATP.

Funding

We thank the Australian Research Council for financial support.

History

School

Science

Department

Chemistry

Published in

CHEMICAL SCIENCE

Volume

4

Issue

4

Pages

1680 - 1686 (7)

Citation

LIU, X. ... et al, 2013. Tuning colourimetric indicator displacement assays for naked-eye sensing of pyrophosphate in aqueous media. Chemical Science, 4 (4), pp. 1680 - 1686.

Publisher

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution 3.0 Unported (CC BY 3.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/by/3.0/

Publication date

2013

Notes

This is an Open Access article published by the Royal Society of Chemistry and licensed under the terms of the Creative Commons Attribution Licence, http://creativecommons.org/licenses/by/3.0/