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Tuning the anion binding properties of lanthanide receptors to discriminate nucleoside phosphates in a sensing array

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posted on 22.03.2021, 14:32 authored by Sarah Hewitt, Georgina Macey, Romain Mailhot, Mark ElsegoodMark Elsegood, Fernanda Duarte, Alan M Kenwright, Stephen ButlerStephen Butler

The development of synthetic receptors for the selective binding and discrimination of anions in water requires an understanding of how anions interact with these synthetic receptors. Molecules designed to differentiate nucleoside phosphate anions (e.g. ATP, ADP, GTP, GDP, UDP) under physiological conditions could underpin exciting new sensing tools for biomedical research and drug discovery, but it is very challenging due to the similarities in anion structure, size and charge. We present a series of lanthanide-based anion receptors and establish key structural elements that impact on nucleoside phosphate anion binding and sensing. Structural evidence of anion binding using X-ray crystallographic and NMR data, supported by DFT calculations indicate the binding modes between the lanthanide complexes and certain phosphoanions, revealing a bidentate (a-, g-) binding mode to ATP. We further use four of the receptors to allow discrimination of eight nucleoside phosphate anions in the first arraybased assay using lanthanide complexes, taking advantage of the multiple emission bands and long emission lifetimes associated with luminescent lanthanide complexes.

Funding

Luminescent Host Molecules for Multisite Recognition of Polyphosphate Anions

Engineering and Physical Sciences Research Council

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Wellcome Trust (204500/Z/16/Z)

History

School

  • Science

Department

  • Chemistry

Published in

Chemical Science

Volume

11

Issue

14

Pages

3619 - 3628

Publisher

Royal Society of Chemistry (RSC)

Version

VoR (Version of Record)

Rights holder

© The authors

Publisher statement

This is an Open Access Article. It is published by Royal Society of Chemistry (RSC) under the Creative Commons Attribution 4.0 Unported Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/

Acceptance date

10/03/2020

Publication date

2020-03-11

Copyright date

2020

ISSN

2041-6520

eISSN

2041-6539

Language

en

Depositor

Dr Stephen Butler. Deposit date: 17 March 2021

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