Use of the ReCIVA device in breath sampling of patients with acute breathlessness: a feasibility study
journal contributionposted on 2020-12-09, 14:10 authored by Karl A Holden, Wadah Ibrahim, Dahlia Salman, Rebecca Cordell, Teresa McNally, Bharti Patel, Rachael Phillips, Caroline Beardsmore, Michael Wilde, Luke Bryant, Amisha Singapuri, Paul Monks, Christofer Brightling, Neil Greening, Paul Thomas, Salman Siddiqui, Erol A. Gaillard
Introduction: Investigating acute multifactorial undifferentiated breathlessness and understanding the driving inflammatory processes can be technically challenging in both adults and children. Being able to validate non-invasive methods such as breath analysis would be a huge clinical advance. The ReCIVA ® device allows breath samples to be collected directly onto sorbent tubes at the bedside for analysis of exhaled volatile organic compounds (eVOCs). We aimed to assess the feasibility of using this device in acutely breathless patients. Methods: Adults hospitalised with acute breathlessness and children aged 5-16 years with acute asthma or chronic stable asthma as well as healthy adult and child volunteers were recruited. Breath samples were collected onto sorbent tubes using the ReCIVA® device and sent for analysis by means of two dimensional gas chromatography-mass spectrometry (GCxGC-MS). The NASA Task Load Index (NASA-TLX) was used to assess the perceived task workload of undertaking sampling from the patients’ perspective. Results: Data was available for 65 adults and 61 children recruited. In total, 98.4% of adults and 75.4% of children were able to provide the full target breath sample using the ReCIVA ® device. NASA TLX measurements was available in the adult population with mean values of 3.37 for effort, 2.34 for frustration, 3.8 for mental demand, 2.8 for performance, 3.9 for physical demand and 2.8 for temporal demand. Discussion: This feasibility study demonstrates it is possible and acceptable to collect breath samples from both adults and children at the bedside for breathomics analysis using the ReCIVA® device.
East Midlands Breathomics Pathology Node (EMBER)
Medical Research CouncilFind out more...