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Vitrified human umbilical arteries as potential grafts for vascular tissue engineering

journal contribution
posted on 25.11.2020, 14:36 by Panagiotis Mallis, Michalis Katsimpoulas, Alkiviadis Kostakis, Daniele Dipresa, Sotiris KorossisSotiris Korossis, Aggeliki Papapanagiotou, Eva Kassi, Catherine Stavropoulos-Giokas, Efstathios Michalopoulos
Background: The development of a biological based small diameter vascular graft (d < 6 mm), that can be properly stored over a long time period at − 196 °C, in order to directly be used to the patients, still remains a challenge. In this study the decellularized umbilical arteries (UAs) where vitrified, evaluated their composition and implanted to a porcine model, thus serving as vascular graft. Methods: Human UAs were decellularized using 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) and sodium dodecyl sulfate (SDS) detergents. Then, vitrified with vitrification solution 55 (VS55) solution, remained for 6 months in liquid nitrogen and their extracellular matrix composition was compared to conventionally cryopreserved UAs. Additionally, total hydroxyproline, sulphated glycosaminoglycan and DNA content were quantified in all samples. Finally, the vitrified umbilical arteries implanted as common carotid artery interposition graft to a porcine animal model. Results: Decellularized and vitrified UAs characterized by proper preservation of extracellular matrix proteins and tissue architecture, whereas conventionally cryopreserved samples exhibited a disorganized structure. Total hydroxyproline content was preserved, although sulphated glycosaminoglycan and DNA contents presented significantly alterations in all samples. Implanted UAs successfully recellularized and remodeled as indicated by the histological analysis. Conclusion: Decellularized and vitrified UAs retained their structure function properties and can be possible used as an alternative source for readily accessible small diameter vascular grafts.

History

School

  • Mechanical, Electrical and Manufacturing Engineering

Published in

Tissue Engineering and Regenerative Medicine

Volume

17

Issue

3

Pages

285 - 299

Publisher

Springer

Version

VoR (Version of Record)

Rights holder

© The Korean Tissue Engineering and Regenerative Medicine Society

Acceptance date

28/01/2020

Publication date

2020-03-13

Copyright date

2020

ISSN

1738-2696

eISSN

2212-5469

Language

en

Depositor

Prof Sotiris Korossis. Deposit date: 24 November 2020