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Volatile atmospheric pressure chemical ionisation mass spectrometry headspace analysis of E. coli and S. aureus

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Identifying the characteristics of bacterial species can improve treatment outcomes and mass spectrometry methods have been shown to be capable of identifying biomarkers of bacterial species. This study is the first to use volatile atmospheric pressure chemical ionisation mass spectrometry to directly and non-invasively analyse the headspace of E. coli and S. aureus bacterial cultures, enabling major biological classification at species level (Gram negative/positive respectively). Four different protocols were used to collect data, three utilising discrete 5 min samples taken between 2 and 96 h after inoculation and one method employing 24 h continuous sampling. Characteristic marker ions were found for both E. coli and S. aureus. A model to distinguish between sample types was able to correctly identify the bacteria samples after sufficient growth (24–48 h), with similar results obtained across different sampling methods. This demonstrates that this is a robust method to analyse and classify bacterial cultures accurately and within a relevant time frame, offering a promising technique for both clinical and research applications

History

School

  • Science
  • Sport, Exercise and Health Sciences

Department

  • Chemistry
  • Mathematical Sciences

Published in

Analytical Methods

Volume

13

Issue

45

Pages

5441-5449

Publisher

Royal Society of Chemistry (RSC)

Version

VoR (Version of Record)

Publisher statement

This is an Open Access Article. It is published by Royal Society of Chemistry under the Creative Commons Attribution-Non Commercial 4.0 International Licence (CC BY-NC). Full details of this licence are available at: https://creativecommons.org/licenses/by-nc/4.0/

Acceptance date

09/11/2021

Publication date

2021-11-15

ISSN

1759-9660

eISSN

1759-9679

Language

en

Depositor

Dr Matthew Turner. Deposit date: 18 November 2021