ChargeRegTransArxiv1.pdf (497.91 kB)
pH-mediated regulation of polymer transport through SiN pores
journal contribution
posted on 2018-10-31, 13:00 authored by Sahin Buyukdagli, Tapio Ala-NissilaTapio Ala-NissilaWe characterize the pH-controlled polymer capture and transport through silicon nitride (SiN) pores subject to protonation. A charge regulation model able to reproduce the experimental zeta potential of SiN pores is coupled with electrohydrodynamic polymer transport equations. The formalism can quantitatively explain the experimentally observed non-monotonic pH dependence of avidin conductivity in terms of the interplay between the electroosmotic and electrophoretic drag forces on the protein. We also scrutinize the DNA conductivity of SiN pores. We show that in the low-pH regime where the amphoteric pore is cationic, DNA-pore attraction acts as an electrostatic trap. This provides a favorable condition for fast polymer capture and extended translocation required for accurate polymer sequencing.
Funding
This work was performed as a part of the Academy of Finland QTF Centre of Excellence program (project 312298) and has also been supported by the Aalto Science Institute through a sabbatical grant (SB).
History
School
- Science
Department
- Mathematical Sciences
Published in
EPLVolume
123Issue
3Citation
BUYUKDAGLI, S. and ALA-NISSILA, T., 2018. pH-mediated regulation of polymer transport through SiN pores. EPL (Europhysics Letters), 123 (3), 38003.Publisher
© EPL AssociationVersion
- VoR (Version of Record)
Publisher statement
This paper was accepted for publication in the journal EPL (Europhysics Letters) and the definitive published version is available at https://doi.org/10.1209/0295-5075/123/38003.Publication date
2018-09-10ISSN
0295-5075eISSN
1286-4854Publisher version
Language
- en