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Aspects of staphylococcal growth, haemolysis and phagocytosis

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thesis
posted on 20.02.2018, 12:11 by Sabah A.A. Jassim
The effects of medium composition on the growth, biochemical and biological activities of selected coagulase positive and negative Staphylococci have been investigated. The study concentrated on four major points, namely: nutrient requirements; haemolysin and enzyme production; their antigenic properties; and phagocytosis of the cells. The nutrient requirements for adequate growth of Staphylococci was investigated in a chemically defined medium (CDM) for each strain to determine the supplemental, required and essential amino acids. It was found that strain/species specific differences in amino acid requirements occur amongst the six Staphylococci. The growth of Staphylococcus epidermidis showed complete biotin-independence. Additionally, media derived from fish waste extract (FE) were prepared after developing a simple rapid method to digest the fish waste. FE was found a good substitute for beef extract in culture media. [Continues.]

History

School

  • Science

Department

  • Chemistry

Publisher

© Sabah A.A. Jassim

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 2.5 Generic (CC BY-NC-ND 2.5) licence. Full details of this licence are available at: http://creativecommons.org/licenses/by-nc-nd/2.5/

Publication date

1989

Notes

A doctoral thesis submitted in partial fulfilment of the requirements for the award of Doctor of Philosophy at Loughborough University.

Language

en

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