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Exploiting resistive pulse sensing for the detection of biomarkers

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thesis
posted on 09.07.2020, 11:20 by Matthew Healey
Having a robust and reliable assay that can be used by clinician inside or outside the hospital laboratory is vital for diagnosing and monitoring the health of a population.The aim of this PhD was to build a foundation of knowledge that could be used to utilise resistive pulse sensing (RPS) coupled with nanoparticles as a platform for biomarker detection. The target of interest, and the overall goal of this thesis, was the prion protein in human blood,which is used for the detection of variant Creutzfeldt–Jakob disease (vCJD). There is no standard detection method for this disease, instead doctors rely on a series of post-mortem tests to determine a diagnosis of an individual. A few assays have been developed, but due to their selectivity issues, and the fact they can take many hours, or days, they are not currently used. This PhD combines the use of RPS with deoxyribonucleic acid(DNA)modified particles as a label free sensor that allows the characterisation of ananalyte bound to DNA modified particles. [Continues.]

History

School

  • Science

Department

  • Chemistry

Publisher

Loughborough University

Rights holder

© Matthew J. Healey

Publication date

2020

Notes

A doctoral thesis. Submitted in partial fulfilment of the requirements for the award of Doctor of Philosophy of Loughborough University.

Language

en

Supervisor(s)

Mark Platt ; Sarabjit Mastana

Qualification name

PhD

Qualification level

Doctoral

This submission includes a signed certificate in addition to the thesis file(s)

I have submitted a signed certificate

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