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Fluorigenic enzyme assays using long-wavelength substrates

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thesis
posted on 31.08.2018, 15:29 by G. Hussain Sarpara
Investigations into the use of long-wavelength fluorigenic substrates, which are non- or weakly fluorescent but are converted by an appropriate enzyme to a highly fluorescent product, have been carried out in order to determine several different enzymes and their inhibitors. Certain nonfluorescent compounds based on long-wavelength xanthenes and oxazines dyes were synthesised. Amongst these, naphthofluorescein diphosphate and naphthofluorescein mono-phosphate has been demonstrated to be new fluorigenic substrates for alkaline phosphatase. The hydrolysis product of these substrates can be used for trace analyses using diode laser based fluorescence detection. [Continues.]

Funding

Pakistan, Ministry of Education.

History

School

  • Science

Department

  • Chemistry

Publisher

© Ghulam Hussain Sarpara

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2000

Notes

A Doctoral Thesis. Submitted in partial fulfilment of the requirements for the award of Doctor of Philosophy at Loughborough University.

Language

en

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