Thesis-1982-Thakrar.pdf (4.3 MB)

New luminescence-linked immunoassay techniques

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thesis
posted on 11.06.2013, 15:16 by Hasumita Thakrar
Several fluorescent labels were studied to develop simple luminescence immunoassay methods for both macromolecules and low molecular weight analytes. Initially, the 'Fluram (fluorescamine) enhancement phenomenon' was investigated by extending its use for assay of the-thyroid hormone, triiodothyronine (T3). The observed enhanced fluorescence permitted the development of a homogeneous assay capable of detecting nanogram concentrations of the hormone in pure solution and hormone depleted serum. The technique was however, complicated by the overlapping Raman scatter peak and attempts to overcome this problem included the use of derivative spectroscopy [continued]…

Funding

Science and Engineering Research Council of Great Britain

History

School

  • Science

Department

  • Chemistry

Publisher

© H. Thakrar

Publication date

1982

EThOS Persistent ID

uk.bl.ethos.290647

Language

en

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